(TH) diazepam binding to human granulocytes
(TH)-diazepam binds to sites on human granulocyte membranes, with little or no binding to platelets or lymphocytes. These (TH)-diazepam binding sites are of the peripheral type, being strongly inhibited by R05-4864 (Ki=6.23nM) but only weakly by clonazepam (Ki=14 M). Binding of (TH) diazepam at 0 is saturable, specific and stereoselective. Scatchard analysis indicates a single class of sites with Bmax of 109 +/- 17f moles per mg of protein and K/sub D/ of 3.07 +/- 0.53nM. Hill plots of saturation experiments gave straight lines with a mean Hill coefficient of 1.03 +/- 0.014. Binding is time dependent and reversible and it varies linearly with granulocyte protein concentration over the range 0.025-0.300 mg of protein. 11 references, 3 figures, 1 table.
- Research Organization:
- St. John's Hospital, Stone, England
- OSTI ID:
- 6335617
- Journal Information:
- Life Sci.; (United States), Vol. 37:1
- Country of Publication:
- United States
- Language:
- English
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TRANQUILIZERS
BIOCHEMICAL REACTION KINETICS
BLOOD PLATELETS
LEUKOCYTES
LYMPHOCYTES
MAN
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CENTRAL NERVOUS SYSTEM AGENTS
CONNECTIVE TISSUE CELLS
DRUGS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
PRIMATES
PSYCHOTROPIC DRUGS
REACTION KINETICS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques