Effect of ethanol on thrombin-induced platelet phospholipid breakdown and release of (TH)-5-hydroxytryptamine
Ethanol has been reported previously to inhibit chemically-induced platelet aggregation and the release of platelet contents. In platelet suspensions the mechanical stimulus of stirring can induce slow aggregation and the loss of endogenous arachidonic acid from phospholipids by activation of platelet phospholipases. These changes are prevented by the presence of ethanol 20-100 mM, whereas, in unstirred suspensions, ethanol alone has no effect on platelet phospholipids. Under similar conditions of reduced platelet: platelet contact, chemical stimuli, such as thrombi, although unable to produce visible aggregation, still cause the release of (TH)-5-hydroxytryptamine from platelets and also initiate the breakdown of platelet phospholipids. Ethanol does not now inhibit the thrombin-induced release of platelet contents and has little effect on phosphatidylinositol breakdown, though it inhibits phosphatidylcholine breakdown. Ethanol may therefore inhibit platelet aggregation by reducing the effect of mechanical and chemical stimuli on the activation of phospholipase A2. In contrast ethanol has rather little effect on the receptor-mediated breakdown of phosphatidylinositol which is apparently sufficient to trigger the release of platelet contents.
- Research Organization:
- Kings College, Strand, London, England
- OSTI ID:
- 6335421
- Journal Information:
- Alcohol; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ETHANOL
BIOLOGICAL EFFECTS
PHOSPHOLIPIDS
DECOMPOSITION
TRITIUM COMPOUNDS
TRACER TECHNIQUES
TRYPTAMINES
BIOCHEMISTRY
BLOOD PLATELETS
IN VITRO
SEROTONIN
THROMBIN
ALCOHOLS
AMINES
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CHEMISTRY
COAGULANTS
DRUGS
ENZYMES
ESTERS
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MATERIALS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HYDROLASES
PYRROLES
RADIOPROTECTIVE SUBSTANCES
SERINE PROTEINASES
SYMPATHOMIMETICS
550201* - Biochemistry- Tracer Techniques