Isolation and chromosomal mapping of the human immunoglobulin-associated B29 gene (IGB)

Journal Article · Thu Apr 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1157· OSTI ID:6333128

Wood, Jr, W J; Thompson, A A; Korenberg, J; Chen, Xianing; May, W; Wall, R; Denny, C T ^[1]

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1. Univ. of California, Los Angeles (United States)

The B29 gene encodes a B-cell-specific membrane protein in the immunoglobulin antigen receptor complex. B29 is a crucial member of this receptor complex and is believed to function as an effector of signal transduction in a manner analogous to that of the CD3 components of the T cell antigen receptor. The authors have isolated a full-length human B29 cDNA clone by using a murine B29 cDNA probe. They show that there is an extremely high degree of evolutionary conservation between the human and mouse proteins, particularly in the transmembrane and intracytoplasmic regions, where the identity is 96%. In addition, the intracytoplasmic region in both proteins contains an identical peptide motif that is present in a number of molecules involved in lymphocyte activation. Genomic Southern blot analysis of human cell lines hybridized with both murine and human B29 cDNAs gives patterns consistent with a single-copy gene occupying a small region of the genomic sequence. Using human B29 cosmid DNA, they have localized the B29 gene to human chromosome 17q23 via fluorescence in situ hybridization. B29 is the first gene localized to this area of the genome. Interestingly, a subset of human B cell chronic lymphocytic leukemias (CLL) has translocations in this locus on chromosome 17. 18 refs., 4 figs.

• OSTI ID: 6333128
• Journal Information: Genomics; (United States), Vol. 16:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 17
MAPPING
LYMPHOCYTES
GENES
MEMBRANE PROTEINS
RECEPTORS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
LEUKEMIA
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
DISEASES
HUMAN CHROMOSOMES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
LEUKOCYTES
MATERIALS
NEOPLASMS
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics