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Title: Preservation of peripheral benzodiazepine receptors: differential effects of freezing on (/sup 3/H)Ro 5-4864 and (3H)PK 11195 binding

Abstract

A statistically significant decrease in the density of peripheral benzodiazepine receptors was observed in renal membranes of rats beginning 2 weeks after adrenalectomy when compared with sham-operated controls. This decrease in peripheral benzodiazepine receptor density was manifest as a decrease in the Bmax of two ligands (/sup 3/H)Ro 5-4864 and (/sup 3/H)PK 11195, without accompanying changes in their apparent affinity (Kd) for this site. Similar changes were not seen in another aldosterone-sensitive organ, the submandibular salivary gland. The decrease in peripheral benzodiazepine receptor density in observed in adrenalectomized rat renal membranes was restored to control levels after 1 week of aldosterone administration using a dose (12.5 micrograms/kg/day) that had no effect on peripheral benzodiazepine receptor density in sham-operated animals. In contrast, dexamethasone administration (50 micrograms/kg/day, 1 week) had no effect on renal peripheral benzodiazepine receptor density when administered to either adrenalectomized or sham-operated rats. Further, adrenal demedullation had no effect on renal peripheral benzodiazepine receptor density or affinity. The decrease in peripheral benzodiazepine receptor density was localized to the renal cortex and the outer stripe of the medulla by gross dissection of renal slices and renal tissue section autoradiography. The specific effect of adrenalectomy on renal peripheral benzodiazepine receptor density,more » the lack of direct effect of aldosterone on (/sup 3/H)Ro 5-4864 binding, and the localization of the change in peripheral benzodiazepine receptor density to the renal cortex and outer stripe suggests that these changes may reflect an adaptation of the renal nephron (possibly the distal convoluted tubule, intermediate tubule and/or the collecting duct) to the loss of mineralocorticoid hormones.« less

Authors:
; ;
Publication Date:
Research Org.:
NIADDK, Bethesda, MD
OSTI Identifier:
6332534
Resource Type:
Journal Article
Journal Name:
J. Pharmacol. Methods; (United States)
Additional Journal Information:
Journal Volume: 2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; RECEPTORS; BIOCHEMICAL REACTION KINETICS; TEMPERATURE EFFECTS; TRANQUILIZERS; ADRENALECTOMY; AFFINITY; ALDOSTERONE; AUTORADIOGRAPHY; CELL MEMBRANES; DEXAMETHASONE; FREEZING; IN VITRO; KIDNEYS; LIGANDS; RATS; TRITIUM COMPOUNDS; ADRENAL HORMONES; ALDEHYDES; ANIMALS; BODY; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM AGENTS; CORTICOSTEROIDS; DRUGS; GLUCOCORTICOIDS; HORMONES; HYDROXY COMPOUNDS; KETONES; KINETICS; LABELLED COMPOUNDS; MAMMALS; MEDICINE; MEMBRANE PROTEINS; MEMBRANES; MINERALOCORTICOIDS; ORGANIC COMPOUNDS; ORGANS; PREGNANES; PROTEINS; PSYCHOTROPIC DRUGS; REACTION KINETICS; RODENTS; STEROID HORMONES; STEROIDS; SURGERY; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 560200 - Thermal Effects

Citation Formats

Basile, A S, Ostrowski, N L, and Skolnick, P. Preservation of peripheral benzodiazepine receptors: differential effects of freezing on (/sup 3/H)Ro 5-4864 and (3H)PK 11195 binding. United States: N. p., 1987. Web. doi:10.1016/0160-5402(87)90025-8.
Basile, A S, Ostrowski, N L, & Skolnick, P. Preservation of peripheral benzodiazepine receptors: differential effects of freezing on (/sup 3/H)Ro 5-4864 and (3H)PK 11195 binding. United States. https://doi.org/10.1016/0160-5402(87)90025-8
Basile, A S, Ostrowski, N L, and Skolnick, P. 1987. "Preservation of peripheral benzodiazepine receptors: differential effects of freezing on (/sup 3/H)Ro 5-4864 and (3H)PK 11195 binding". United States. https://doi.org/10.1016/0160-5402(87)90025-8.
@article{osti_6332534,
title = {Preservation of peripheral benzodiazepine receptors: differential effects of freezing on (/sup 3/H)Ro 5-4864 and (3H)PK 11195 binding},
author = {Basile, A S and Ostrowski, N L and Skolnick, P},
abstractNote = {A statistically significant decrease in the density of peripheral benzodiazepine receptors was observed in renal membranes of rats beginning 2 weeks after adrenalectomy when compared with sham-operated controls. This decrease in peripheral benzodiazepine receptor density was manifest as a decrease in the Bmax of two ligands (/sup 3/H)Ro 5-4864 and (/sup 3/H)PK 11195, without accompanying changes in their apparent affinity (Kd) for this site. Similar changes were not seen in another aldosterone-sensitive organ, the submandibular salivary gland. The decrease in peripheral benzodiazepine receptor density in observed in adrenalectomized rat renal membranes was restored to control levels after 1 week of aldosterone administration using a dose (12.5 micrograms/kg/day) that had no effect on peripheral benzodiazepine receptor density in sham-operated animals. In contrast, dexamethasone administration (50 micrograms/kg/day, 1 week) had no effect on renal peripheral benzodiazepine receptor density when administered to either adrenalectomized or sham-operated rats. Further, adrenal demedullation had no effect on renal peripheral benzodiazepine receptor density or affinity. The decrease in peripheral benzodiazepine receptor density was localized to the renal cortex and the outer stripe of the medulla by gross dissection of renal slices and renal tissue section autoradiography. The specific effect of adrenalectomy on renal peripheral benzodiazepine receptor density, the lack of direct effect of aldosterone on (/sup 3/H)Ro 5-4864 binding, and the localization of the change in peripheral benzodiazepine receptor density to the renal cortex and outer stripe suggests that these changes may reflect an adaptation of the renal nephron (possibly the distal convoluted tubule, intermediate tubule and/or the collecting duct) to the loss of mineralocorticoid hormones.},
doi = {10.1016/0160-5402(87)90025-8},
url = {https://www.osti.gov/biblio/6332534}, journal = {J. Pharmacol. Methods; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Wed Apr 01 00:00:00 EST 1987},
month = {Wed Apr 01 00:00:00 EST 1987}
}