Lymphoscintigraphy of human colorectal carcinoma metastases in athymic mice by use of radioiodinated B72. 3 monoclonal antibody

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · J. Natl. Cancer Inst.; (United States)

OSTI ID:6331781

Shah, S A; Gallagher, B M; Sands, H

The potential of radioiodinated monoclonal antibody B72.3 for lymphoscintigraphy was evaluated, using suitable animal models of a human colorectal carcinoma. LS174T xenografts were grown at various sites in beta-estradiol-pretreated athymic mice, and the development of metastases in different organs was assessed histologically. After iv inoculation of the mice, 66% of the animals developed ''metastases'' to the axillary lymph nodes. Of these mice, 100% also developed multiple tumors on their backs and 79% had lung micrometastases. Livers, kidneys, and spleens showed no evidence of tumor growth. In 33% of the mice in which primary LS174T tumors had been removed from the hindfoot pad, metastases to the popliteal lymph nodes were observed 3 1/2 weeks after tumor implantation. BALB/c (nu/nu) female mice bearing axillary and popliteal lymph node metastases were used to test the potential of radiolabeled B72.3 antibody (an IgG1) as a lymphoscintigraphic agent. A monoclonal antibody against horseradish peroxidase (also an IgG1), which did not bind LS174T tumor cells in vitro, served as a control. Both normal and tumor-bearing axillary and popliteal lymph nodes imaged up to 6 hours after the sc injection of 20-40 mu Ci of /sup 125/I-labeled B72.3 into either the forefoot or hindfoot pads. The localization index (L.I.) (specific/nonspecific antibody in tumor divided by specific/nonspecific antibody in blood) for LS174T tumors in lymph nodes was approximately 1 during the first 6 hours after antibody injection, thus indicating no specific antibody accumulation.

• Research Organization: E.I. du Pont de Nemours and Co., Inc., North Billerica, MA
• OSTI ID: 6331781
• Journal Information: J. Natl. Cancer Inst.; (United States), Vol. 6
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
GASTROINTESTINAL TRACT
CARCINOMAS
LYMPH NODES
SCINTISCANNING
METASTASES
DIAGNOSIS
MONOCLONAL ANTIBODIES
DIAGNOSTIC USES
AUTORADIOGRAPHY
BIOLOGICAL MODELS
IODINE 125
MICE
TRANSPLANTS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LYMPHATIC SYSTEM
MAMMALS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics