Regulation and genetic organization of hydrogenase: Progress report, 1 June 1985--31 March 1986

Technical Report · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6327207

Krasna, A I

The experiments concentrate on development of screening methods and cloning of the hydrogenase gene. The first method used to distinguish between hydrogenase-negative and hydrogenase-positive cells of E. coli is to measure the ability of colonies of the cells on agar plates (with complex or minimal media with or without antibiotics) to reduce the dye methyl viologen with H/sub 2/ gas. The presence of hydrogenase activity in the positive colonies is confirmed by growth in liquid media. 3 figs., 2 tabs.

• Research Organization: Columbia Univ., New York (USA). Coll. of Physicians and Surgeons
• DOE Contract Number: FG02-85ER13374
• OSTI ID: 6327207
• Report Number(s): DOE/ER/13374-1; ON: DE89008461
• Resource Relation: Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESCHERICHIA COLI
METABOLISM
HYDROGENASES
EVALUATION
ANAEROBIC CONDITIONS
DNA-CLONING
FORMATES
GENE REGULATION
GENETIC CONTROL
GENETIC MAPPING
MICROORGANISMS
MUTATIONS
PROGRESS REPORT
BACTERIA
CARBOXYLIC ACID SALTS
CLONING
CONTROL
DNA HYBRIDIZATION
DOCUMENT TYPES
ENZYMES
HYBRIDIZATION
MAPPING
OXIDOREDUCTASES
PEST CONTROL
550200* - Biochemistry
550400 - Genetics