Lipid distribution and phospholipase A/sub 2/ (PLA/sub 2/) activity in skeletal muscle from malignant hyperthermia (MH) susceptible and normal pigs
The authors examined the lipid distribution and PLA2 activity of skeletal muscle from three pigs in each of three phenotypic groups. The three groups did not differ in respect to total FFA, cholesterol, triglycerides, or total lipid phosphorus. The distributions of phospholipids, as determined by 2-D TLC and phosphorus quantification, and fatty acids, as determined by 1-D TLC and GC, were also similar. When muscle homogenates were incubated with radiolabeled Triton/phosphatidylcholine mixed micelles to measure PLA2 activity, three groups could be distinguished by percent of total radiolabel that was FFA: MH+, 4.9 +/- 0.3%; MH +/-, 3.4 +/- 0.3%; MH-, 1.0 +/- 0.1%. Therefore, MH susceptible pigs differ from normals in having elevated PLA2 activity, which is evident in incubated muscle homogenates, but not intact tissue.
- Research Organization:
- Hahnemann Univ., Philadelphia, PA
- OSTI ID:
- 6326157
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPASE
ENZYME ACTIVITY
LIPIDS
TISSUE DISTRIBUTION
SWINE
SENSITIVITY
GAS CHROMATOGRAPHY
GENETIC VARIABILITY
MUSCLES
PHENOTYPE
PHOSPHOLIPIDS
RADIOISOTOPES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
ANIMALS
BIOLOGICAL VARIABILITY
CARBOXYLESTERASES
CHROMATOGRAPHY
DISTRIBUTION
DOMESTIC ANIMALS
ENZYMES
ESTERASES
ESTERS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques