Histochemical and structural analysis of mucous glycoprotein secreted by the gill of Mytilus edulis
Studies were carried out to characterized various mucous cells in the gill filament, to ascertain structural characteristics of the secreted mucous glycoproteins, and to determine the ability of the gill epithelium to incorporate ({sup 14}C)glucosamine as a precursor in the biosynthesis and secretion of mucous glycoproteins. Using histochemical staining techniques, mucous cells containing neutral and acidic mucins were found in the lateral region, whereas mucous cells containing primarily neutral or sulfated mucins were found in the postlateral region. Serotonin, but not dopamine, stimulated the mucous secretion. In tissues pretreated with ({sup 14}C)glucosamine, the secreted glycoproteins contain incorporated radiolabel. Analysis by column chromatography using Bio-Gel P-2 and P-6 shows that the secretion contains two glycoprotein populations. Glycoprotein II has a molecular weight of 2.3 {times} 10{sup 4} daltons. Upon alkaline reductive borohydride cleavage of the O-glycosidic linkages of glycoprotein I, about 70% of the radiolabel was removed from the protein. Gas chromatographic analysis of the carbohydrate composition shows that the glycoproteins contains N-acetylglucosamine (GluNAc), N-acetylgalactosamine (GalNAc), and galactose, fucose and mannose. Amino acid analysis shows that the glycoproteins are rich in serine, threonine and proline.
- Research Organization:
- West Virginia Univ., Morgantown, WV (USA)
- OSTI ID:
- 6325504
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLYCOPROTEINS
MOLECULAR STRUCTURE
AMINO ACID SEQUENCE
BIOSYNTHESIS
CARBON 14 COMPOUNDS
GAS CHROMATOGRAPHY
GILLS
GLUCOSAMINE
MOLECULAR WEIGHT
MUSSELS
SECRETION
SEROTONIN
TRACER TECHNIQUES
AMINES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
CARBOHYDRATES
CHROMATOGRAPHY
DRUGS
HETEROCYCLIC COMPOUNDS
HEXOSAMINES
HEXOSES
HYDROXY COMPOUNDS
INDOLES
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MOLLUSCS
MONOSACCHARIDES
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
SACCHARIDES
SEPARATION PROCESSES
SYMPATHOMIMETICS
SYNTHESIS
TRYPTAMINES
550201* - Biochemistry- Tracer Techniques