( sup 14 C)mechlorethamine binding to proteins of the human keratinocyte
- Letterman Army Institute of Research, Presidio of San Francisco, CA (USA)
Much mustard agent research has focused on mustard/DNA interactions. Mustard also interacts with proteins, however, and to reach the DNA any agent must first pass through the cytoplasm. We hypothesized that the cell's proteins would covalently bind mustard, and thereby limit its access to the DNA. Keratinocyte proteins were radiolabeled with ({sup 14}C)mechlorethamine and separated by electrophoresis. The banding patterns that resulted were made visible on x-ray films, then compared with control patterns. A correspondence of almost one-to-one was observed, which supports the hypothesis that many cellular proteins are susceptible to mustard alkylation. It follows that some mustard symptoms probably result from effects on existing proteins.
- OSTI ID:
- 6324790
- Journal Information:
- Military Medicine; (USA), Vol. 155:10; ISSN 0026-4075
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NITROGEN MUSTARD
BIOCHEMICAL REACTION KINETICS
PROTEINS
ALKYLATION
ANIMAL CELLS
CARBON 14 COMPOUNDS
DNA
ELECTROPHORESIS
EPIDERMIS
MAN
TRACER TECHNIQUES
ALKYLATING AGENTS
AMINES
ANIMAL TISSUES
ANIMALS
BODY
CHEMICAL REACTIONS
EPITHELIUM
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PRIMATES
REACTION KINETICS
SKIN
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques