Localization of phosphatidylinositol signaling components in rat taste cells: Role in bitter taste transduction

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.19.7395· OSTI ID:6323896

Hwang, P M; Verma, A; Bredt, D S; Snyder, S H ^[1]

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1. Johns Hopkins Univ. School of Medicine, Baltimore, MD (USA)

To assess the role of phosphatidylinositol turnover in taste transduction we have visualized, in rat tongue, ATP-dependent endoplasmic reticular accumulation of {sup 45}Ca{sup 2+}, inositol 1,4,5-trisphosphate receptor binding sites, and phosphatidylinositol turnover monitored by autoradiography of ({sup 3}H)cytidine diphosphate diacylglycerol formed from ({sup 3}H)cytidine. Accumulated {sup 45}Ca{sup 2+}, inositol 1,4,5-trisphosphate receptors, and phosphatidylinositol turnover are selectively localized to apical areas of the taste buds of circumvallate papillae, which are associated with bitter taste. Further evidence for a role of phosphatidylinositol turnover in bitter taste is our observation of a rapid, selective increase in mass levels of inositol 1,4,5-trisphosphate elicited by low concentrations of denatonium, a potently bitter tastant.

• OSTI ID: 6323896
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:19; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOLIPIDS
METABOLISM
RECEPTORS
BIOLOGICAL LOCALIZATION
TASTE BUDS
AUTORADIOGRAPHY
CALCIUM 45
CYTIDINE
ENDOPLASMIC RETICULUM
RATS
TONGUE
TRITIUM COMPOUNDS
ALKALINE EARTH ISOTOPES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ESTERS
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LIPIDS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORAL CAVITY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RODENTS
SENSE ORGANS
VERTEBRATES
550501* - Metabolism- Tracer Techniques