sup 31 P NMR measurements of the ADP concentration in yeast cells genetically modified to express creatine kinase

Journal Article · Tue Apr 03 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00465a021· OSTI ID:6323317

Brindle, K; Braddock, P; Fulton, S ^[1]

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1. Univ. of Oxford (England)

Rabbit muscle creatine kinase has been introduced into the yeast Saccharomyces cerevisiae by transforming cells with a multicopy plasmid containing the coding sequence for the enzyme under the control of the yeast phosphoglycerate kinase promoter. The transformed cells showed creating kinase activities similar to those found in mammalian heart muscle. {sup 31}P NMR measurements of the near-equilibrium concentrations of phosphocreatine and cellular pH together with measurements of the total extractable concentrations of phosphocreatine and creatine allowed calculation of the free ADP/ATP ratio in the cell. The calculated ratio of approximately 2 was considerably higher than the ratio of between 0.06 and 0.1 measured directly in cell extracts.

• OSTI ID: 6323317
• Journal Information: Biochemistry; (USA), Vol. 29:13; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ADP
NUCLEAR MAGNETIC RESONANCE
PHOSPHOCREATINE
BIOCHEMISTRY
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
ATP
CREATINE
MUSCLES
PH VALUE
PHOSPHORUS 31
PLASMIDS
RABBITS
SACCHAROMYCES CEREVISIAE
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
ENZYMES
EUMYCOTA
FUNGI
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
RESONANCE
SACCHAROMYCES
STABLE ISOTOPES
TRANSFERASES
VERTEBRATES
YEASTS
550601* - Medicine- Unsealed Radionuclides in Diagnostics