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Title: Site and strand specificity of UVB mutagenesis in the SUP4-o gene of yeast

Abstract

DNA sequencing was used to characterize 208 mutations induced in the SUP4-o tRNA gene of the yeast Saccharomyces cerevisiae by UVB (285-320 nm) radiation. The results were compared to those for an analysis of 211 SUP4-o mutations induced by 254-nm UVC light. In each case, greater than 90% of the mutations were single base-pair changes but G.C----A.T transitions predominated and accounted for more of the mutations induced by UVB than UVC. Double substitutions, single base-pair deletions, and more complex events were also recovered. However, UVB induced 3-fold more tandem substitutions than UVC and nontandem double events were detected only after irradiation with UVC. Virtually all induced substitutions occurred at sites where the pyrimidine of the base pair was part of a dipyrimidine sequence. Although the site specificities were consistent with roles for cyclobutane dimers and pyrimidine-pyrimidone(6-4) lesions in mutation induction, preliminary photoreactivation data implicated cyclobutane dimers as the major form of premutational DNA damage for both agents. Intriguingly, there was a preference for both UVB- and UVC-induced mutations to occur at sites where the dipyrimidine was on the transcribed strand.

Authors:
;  [1]
  1. Univ. of Manitoba, Winnipeg (Canada)
Publication Date:
OSTI Identifier:
6320757
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 87:22; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; GENE MUTATIONS; RADIOINDUCTION; SACCHAROMYCES CEREVISIAE; GENETIC RADIATION EFFECTS; TRANSFER RNA; GENES; ULTRAVIOLET RADIATION; MUTAGENESIS; DNA REPAIR; DNA SEQUENCING; PYRIMIDINE DIMERS; TRANSCRIPTION; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; ELECTROMAGNETIC RADIATION; EUMYCOTA; FUNGI; GENETIC EFFECTS; MICROORGANISMS; MUTATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; RNA; SACCHAROMYCES; STRUCTURAL CHEMICAL ANALYSIS; YEASTS; 560130* - Radiation Effects on Microorganisms

Citation Formats

Armstrong, J D, and Kunz, B A. Site and strand specificity of UVB mutagenesis in the SUP4-o gene of yeast. United States: N. p., 1990. Web. doi:10.1073/pnas.87.22.9005.
Armstrong, J D, & Kunz, B A. Site and strand specificity of UVB mutagenesis in the SUP4-o gene of yeast. United States. https://doi.org/10.1073/pnas.87.22.9005
Armstrong, J D, and Kunz, B A. 1990. "Site and strand specificity of UVB mutagenesis in the SUP4-o gene of yeast". United States. https://doi.org/10.1073/pnas.87.22.9005.
@article{osti_6320757,
title = {Site and strand specificity of UVB mutagenesis in the SUP4-o gene of yeast},
author = {Armstrong, J D and Kunz, B A},
abstractNote = {DNA sequencing was used to characterize 208 mutations induced in the SUP4-o tRNA gene of the yeast Saccharomyces cerevisiae by UVB (285-320 nm) radiation. The results were compared to those for an analysis of 211 SUP4-o mutations induced by 254-nm UVC light. In each case, greater than 90% of the mutations were single base-pair changes but G.C----A.T transitions predominated and accounted for more of the mutations induced by UVB than UVC. Double substitutions, single base-pair deletions, and more complex events were also recovered. However, UVB induced 3-fold more tandem substitutions than UVC and nontandem double events were detected only after irradiation with UVC. Virtually all induced substitutions occurred at sites where the pyrimidine of the base pair was part of a dipyrimidine sequence. Although the site specificities were consistent with roles for cyclobutane dimers and pyrimidine-pyrimidone(6-4) lesions in mutation induction, preliminary photoreactivation data implicated cyclobutane dimers as the major form of premutational DNA damage for both agents. Intriguingly, there was a preference for both UVB- and UVC-induced mutations to occur at sites where the dipyrimidine was on the transcribed strand.},
doi = {10.1073/pnas.87.22.9005},
url = {https://www.osti.gov/biblio/6320757}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 87:22,
place = {United States},
year = {Thu Nov 01 00:00:00 EST 1990},
month = {Thu Nov 01 00:00:00 EST 1990}
}