Fluoride stimulates ( sup 3 H)thymidine incorporation and alkaline phosphatase production by human osteoblasts

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Metabolism, Clinical and Experimental; (USA)

DOI:https://doi.org/10.1016/0026-0495(90)90081-M· OSTI ID:6318062

Khokher, M A; Dandona, P ^[1]

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1. Royal Free Hospital, London (England)

The effect of sodium fluoride on alkaline phosphatase (ALP) release and ({sup 3}H)thymidine uptake by human osteoblasts in culture was investigated. Sodium fluoride stimulated both ALP release and ({sup 3}H)thymidine uptake at concentrations of sodium fluoride greater than 250 mumol/L. This stimulation was similar in magnitude to that induced by 1,25-dihydroxycholecalciferol. The fluoride-induced increase in ALP was inhibited by verapamil, a calcium channel blocker. We conclude that sodium fluoride stimulates osteoblasts to proliferate and to release ALP. This stimulation by fluoride is dependent on calcium influx. Fluoride-induced stimulation of human osteoblasts may be relevant to its effect in enhancing bone formation in patients with osteoporosis.

• OSTI ID: 6318062
• Journal Information: Metabolism, Clinical and Experimental; (USA), Vol. 39:11; ISSN 0026-0495
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
BIOSYNTHESIS
BONE CELLS
CELL PROLIFERATION
FLUORIDES
BIOLOGICAL EFFECTS
OSTEOPOROSIS
THERAPY
THYMIDINE
CELL CULTURES
MAN
PORINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
CONNECTIVE TISSUE CELLS
DISEASES
ENZYMES
ESTERASES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANE PROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATASES
PRIMATES
PROTEINS
PYRIMIDINES
RIBOSIDES
SKELETAL DISEASES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550901 - Pathology- Tracer Techniques