Diminished osmotic fragility of human erythrocytes following the membrane insertion of oxygenated sterol compounds

Journal Article · Sat Aug 01 00:00:00 EDT 1981 · Blood; (United States)

OSTI ID:6315661

Streuli, R A; Kanofsky, J R; Gunn, R B; Yachnin, S

Oxygenated sterol compounds (OSC), when incubated for 1 hr with human erythrocytes in lipoprotein-depleted medium at concentrations of 0.625-5 x 10/sup -5/M, are inserted into the cell membrane and remain there despite subsequent washing of the cells. The insertion results in expansion of the surface area of the red cell ghost membrane, an increase in critical hemolytic volume, and as a consequence, in dimished osmotic fragility of the erythrocytes. This effect is seen with echinocyte-forming as well as with non-echinocyte-forming OSC. Erythrocytes treated with OSC do not differ from control cells with respect to their mean cell volume (MCV) in isotonic solution, water content, ion fluxes, and filterability through polycarbonate filters. The shift of the osmotic fragility curve toward lower NaCl concentrations is proportional to the amount of OSC inserted into the red cell membrane. 7BETA-Hydroxycholesterol, 22-ketocholesterol, and 20 ..cap alpha..-hydroxycholesterol are the most potent inhibitors of osmotic lysis. The effect of OSC on osmotic fragility is diminished if the erythrocytes are incubated in a lipoprotein-containing medium; free cholesterol, however, does not change this effect. Various progesterones also protect red cells from osmotic lysis if the erythrocytes are directly exposed to the compounds present in the hypotonic NaCl solutions.

• Research Organization: Univ. of Chicago, IL
• DOE Contract Number: AC02-80EV10357
• OSTI ID: 6315661
• Journal Information: Blood; (United States), Vol. 58:2; Conference: 23. annual meeting of American Society of Hematology, San Antonio, TX, USA, 5 Dec 1981
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ERYTHROCYTES
SENSITIVITY
STEROLS
BIOLOGICAL EFFECTS
CELL MEMBRANES
CHEMOTHERAPY
CULTURE MEDIA
HEMIC DISEASES
LIPOPROTEINS
OSMOSIS
PHARMACOLOGY
SURFACE AREA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
DIFFUSION
DISEASES
HYDROXY COMPOUNDS
LIPIDS
MATERIALS
MEMBRANES
ORGANIC COMPOUNDS
PROTEINS
STEROIDS
SURFACE PROPERTIES
THERAPY
550300* - Cytology
550600 - Medicine