Molecular biological enhancement of coal desulfurization: Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in Pseudomonads and Thiobacillae

Technical Report · Mon Oct 22 00:00:00 EDT 1990

OSTI ID:6309219

Krawiec, S

Research continued on coal desulfurization and the study of biological enhancement of desulfurization. This quarters work included: crosses between soil isolates containing r68.45 and P. aeruginosa 27853-2a; extents of crosses and abundances of transconjugants; purpose of repeated crosses; noteworthy features of mating protocol; phenotypic peculiarities and phenotypic stabilities of transconjugants; characterization of antibiotic sensitivity; effect of receiving R68.45 from P. putida PRS 2003; transfer of R68.45 from C18 to auxotrophs derived from P. aeruginosa 27853; and differences in ability of various soil isolates to transfer Dbt{sup +} phenotype to recipient cells; incipient characterizations of Thiobacillae spp. growth and antibiotic sensitivity; plasmid isolation; and acquisition of new soil isolates.

• Research Organization: Lehigh Univ., Bethlehem, PA (USA). Dept. of Biology
• Sponsoring Organization: DOE/FE
• DOE Contract Number: AC22-89PC89903
• OSTI ID: 6309219
• Report Number(s): DOE/PC/89903-T1; ON: DE91007448
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
59 BASIC BIOLOGICAL SCIENCES
BACILLUS
GENES
COAL
DESULFURIZATION
PSEUDOMONAS
ANTIBIOTICS
BIOCONVERSION
CLONING
CROSSING-OVER
CULTURE MEDIA
PHENOTYPE
PROGRESS REPORT
SOILS
ANTI-INFECTIVE AGENTS
BACTERIA
CARBONACEOUS MATERIALS
CHEMICAL REACTIONS
DOCUMENT TYPES
DRUGS
ENERGY SOURCES
FOSSIL FUELS
FUELS
MATERIALS
MICROORGANISMS
010402* - Coal
Lignite
& Peat- Purification & Upgrading
550700 - Microbiology
550400 - Genetics