Depression of afferent arc of the in vivo cytotoxic T-cell immunity by bacterial lipopolysaccharides

Journal Article · Tue Oct 15 00:00:00 EDT 1985 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(85)90320-X· OSTI ID:6299014

Mizoguchi, K; Nakashima, I; Hasegawa, Y; Isobe, K; Kato, N; Shimokata, K; Kawashima, K; Nagase, F; Ando, K; Yoshida, T

The afferent arc of the in vivo cytotoxic T-cell immunity assessed by second set rejection of ascitic allogeneic tumors was shown to be depressed by bacterial lipopolysaccharide (LPS) that was administered simultaneously with or 1 day before injection of allogeneic spleen cells as stimulators. Two different LPSs from Escherichia coli O55 and Klebsiella O3 displayed similar activities whereas dextran sulfate, concanavalin A, or poly A:U was not effective. Stimulator activities of allogeneic cells was not directly modified by LPS. Any definite suppressor activity on afferent or efferent arc of the T-cell response was not demonstrable in mice receiving LPS and allogeneic cells. Further, the LPS effect for immune depression was not diminished by whole body X-ray irradiation to the recipient at 300 R, which ablated the B-cell reactivity to LPS for polyclonal activation, or by treatment of the recipient with carrageenan, a known toxic agent to macrophages. It was suggested from these results that LPS suppresses the cytotoxic T-cell immunity by modulating responder T cells to be temporarily refractory to the allogeneic stimulus rather than by activating suppressor cells such as radiation-sensitive lymphocytes and carrageenan-sensitive macrophages.

• Research Organization: Nagoya Univ. School of Medicine, Aichi, Japan
• OSTI ID: 6299014
• Journal Information: Cell. Immunol.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LIPOPOLYSACCHARIDES
BIOLOGICAL EFFECTS
LYMPHOCYTES
IMMUNITY
IMMUNOSUPPRESSION
MICE
RADIATION CHIMERAS
SPLEEN CELLS
WHOLE-BODY IRRADIATION
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CHIMERAS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
IONIZING RADIATIONS
IRRADIATION
LEUKOCYTES
LIPIDS
MAMMALS
MATERIALS
MOSAICISM
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIATIONS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals