Ethanol production by thermophilic bacteria: fermentation of cellulosic substrates by cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum
Abstract
The fermentation of various saccharides derived from cellulosic biomass to ethanol was examined in mono- and cocultures of Clostridium thermocellum strain LQRI and C. thermohydrosulfuricum strain 39E. C. thermohydrosulfuricum fermented glucose, cellobiose, and xylose, but not cellulose or xylan, and yielded ethanol/acetate ratios of >7.0 C. thermocellum fermented a variety of cellulosic substrates, glucose, and cellobiose, but not xylan or xylose, and yielded ethanol/acetate ratios of approx. 1.0. A stable coculture that contained nearly equal numbers of C. thermocellum and C. thermohydrosulfuricum was established that fermented a variety of cellulosic substrates, and the ethanol yield observed was twofold higher than in C. thermocellum monoculture fermentations. The metabolic basis for the enhanced fermentation effectiveness of the coculture on Solka Floc cellulose included: the ability of C. thermocellum cellulase to hydrolyze ..cap alpha..-cellulose and hemicellulose; the enhanced utilization of mono- and disaccharides by C. thermohydrosulfuricum; increased cellulose consumption; threefold increase in the ethanol production rate; and twofold decrease in the acetate production rate.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison
- OSTI Identifier:
- 6298673
- Resource Type:
- Journal Article
- Journal Name:
- Appl. Environ. Microbiol.; (United States)
- Additional Journal Information:
- Journal Volume: 41:6
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CELLOBIOSE; FERMENTATION; CELLULOSE; GLUCOSE; XYLANS; XYLOSE; CLOSTRIDIUM; ETHANOL; ALCOHOLS; ALDEHYDES; BACTERIA; BIOCONVERSION; CARBOHYDRATES; DISACCHARIDES; HEMICELLULOSE; HEXOSES; HYDROXY COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; POLYSACCHARIDES; SACCHARIDES; 550700* - Microbiology; 550200 - Biochemistry
Citation Formats
Ng, T K, Ben-Bassat, A, and Zeikus, J G. Ethanol production by thermophilic bacteria: fermentation of cellulosic substrates by cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum. United States: N. p., 1981.
Web.
Ng, T K, Ben-Bassat, A, & Zeikus, J G. Ethanol production by thermophilic bacteria: fermentation of cellulosic substrates by cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum. United States.
Ng, T K, Ben-Bassat, A, and Zeikus, J G. 1981.
"Ethanol production by thermophilic bacteria: fermentation of cellulosic substrates by cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum". United States.
@article{osti_6298673,
title = {Ethanol production by thermophilic bacteria: fermentation of cellulosic substrates by cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum},
author = {Ng, T K and Ben-Bassat, A and Zeikus, J G},
abstractNote = {The fermentation of various saccharides derived from cellulosic biomass to ethanol was examined in mono- and cocultures of Clostridium thermocellum strain LQRI and C. thermohydrosulfuricum strain 39E. C. thermohydrosulfuricum fermented glucose, cellobiose, and xylose, but not cellulose or xylan, and yielded ethanol/acetate ratios of >7.0 C. thermocellum fermented a variety of cellulosic substrates, glucose, and cellobiose, but not xylan or xylose, and yielded ethanol/acetate ratios of approx. 1.0. A stable coculture that contained nearly equal numbers of C. thermocellum and C. thermohydrosulfuricum was established that fermented a variety of cellulosic substrates, and the ethanol yield observed was twofold higher than in C. thermocellum monoculture fermentations. The metabolic basis for the enhanced fermentation effectiveness of the coculture on Solka Floc cellulose included: the ability of C. thermocellum cellulase to hydrolyze ..cap alpha..-cellulose and hemicellulose; the enhanced utilization of mono- and disaccharides by C. thermohydrosulfuricum; increased cellulose consumption; threefold increase in the ethanol production rate; and twofold decrease in the acetate production rate.},
doi = {},
url = {https://www.osti.gov/biblio/6298673},
journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 41:6,
place = {United States},
year = {Mon Jun 01 00:00:00 EDT 1981},
month = {Mon Jun 01 00:00:00 EDT 1981}
}