Insulin binding properties of normal and transformed human epidermal cultured keratinocytes

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12276922· OSTI ID:6297605

Verrando, P; Ortonne, J P

Insulin binding to its receptors was studied in cultured normal and transformed (A431 line) human epidermal keratinocytes. The specific binding was a temperature-dependent, saturable process. Normal keratinocytes possess a mean value of about 80,000 receptors per cell. Fifteen hours exposure of the cells to insulin lowered their receptor number (about 65% loss in available sites); these reappeared when the hormone was removed from the culture medium. In the A431 epidermoid carcinoma cell line, there is a net decrease in insulin binding (84% of the initial bound/free hormone ratio in comparison with normal cells) essentially related to a loss in receptor affinity for insulin. Thus, cultured human keratinocytes which express insulin receptors may be a useful tool in understanding skin pathology related to insulin disorders.

• Research Organization: Laboratoire de Recherches Dermatologiques, Nice, France
• OSTI ID: 6297605
• Journal Information: J. Invest. Dermatol.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INSULIN
RADIORECEPTOR ASSAY
RECEPTORS
BIOCHEMISTRY
CARCINOMAS
EPIDERMIS
IODINE ISOTOPES
KERATIN
SKIN
TUMOR CELLS
ANIMAL CELLS
ANIMAL TISSUES
BODY
CHEMISTRY
DISEASES
EPITHELIUM
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PROTEINS
SCLEROPROTEINS
TISSUES
TRACER TECHNIQUES
550301* - Cytology- Tracer Techniques