(/sup 3/H)pirenzepine selectively identifies a high affinity population of muscarinic cholinergic receptors in the rat cerebral cortex

Journal Article · Mon Nov 01 00:00:00 EST 1982 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(82)90041-8· OSTI ID:6295759

Watson, M; Roeske, W R; Yamamura, H I

The specific binding of (/sup 3/H)pirenzepine was investigated in homogenates of rat cerebral cortex, cerebellar cortex, and heart. Specific binding of (/sup 3/H)pirenzepine in the cerebral cortex as defined by displacement with atropine sulfate (1..mu..M) was of high affinity (K/sub d/ = 4-10 nM, receptor density = 1.06 pmoles/mg protein), stereoselective, and competitive with drugs specific for the muscarinic receptor. In contrast, few (/sup 3/H)pirenzepine binding sites were demonstrated in cerebellar and heart homogenates.

• OSTI ID: 6295759
• Journal Information: Life Sci.; (United States), Vol. 31:18
• Country of Publication: United States
• Language: English

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