PTH (parathyroid hormone) elevates inositol polyphosphates and diacylglycerol in a rat osteoblast-like cell line

Journal Article · Tue Nov 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6291571

Civitelli, R; Reid, I R; Westbrook, S; Avioli, L V; Hruska, K A ^[1]

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1. Jewish Hospital of St. Louis, MO (USA)

Parathyroid hormone (PTH)-stimulated signal transduction through mechanisms alternate to adenosine 3{prime},5{prime}-cyclic monophosphate (cAMP) production were studied in UMR 106-01 cells, a cell line with an osteoblastic phenotype. PTH produced transient, dose-related increases in cytosolic calcium ((Ca{sup 2+}){sub i}), inositol trisphosphates, and diacylglycerol (DAG). Both inositol 1,4,5-trisphosphate (Ins-1,4,5P{sub 3}) and inositol 1,3,4-trisphosphate (Ins-1,3,4P{sub 3}) production were rapidly stimulated by PTH. Consistent with the production of Ins-1,3,4P{sub 3}, rapid stimulation of late eluting inositol tetrakisphosphate was observed. The effects on the inositol phosphates were induced rapidly, consistent with roles as signals for changes in (Ca{sup 2+}){sub i}. In saponin-permeabilized UMR 106-01 cells, Ins-1,4,5P{sub 3} stimulated {sup 45}Ca release from a nonmitochondrial intracellular pool. Thus the hypothesis that PTH-stimulated Ins-1,4,5P{sub 3} production initiates Ca{sup 2+} release and contributes to transient elevations of (Ca{sup 2+}){sub i} is supported. These data suggest that stimulation of cAMP production during PTH stimulation may negatively affect production of rises in (Ca{sup 2+}){sub i} during PTH stimulation. The inactivation of the inhibitory G protein of adenylate cyclase by pertussis toxin could explain its action similar to cAMP analogues. Cyclci nucleotides diminish the effects of PTH on (Ca{sup 2+}){sub i}, probably interacting on a biochemical step subsequent to or independent of Ins-1,4,5P{sub 3} release.

• OSTI ID: 6291571
• Journal Information: American Journal of Physiology; (USA), Vol. 255:5; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM 45
UPTAKE
GLYCEROL
BIOCHEMISTRY
PARATHORMONE
BIOLOGICAL EFFECTS
PHOSPHORIC ACID ESTERS
AMP
ARACHIDONIC ACID
BONE CELLS
CALCIUM CHLORIDES
INOSITOL
OSTEOSARCOMAS
PERMEABILITY
TRITIUM COMPOUNDS
TUMOR CELLS
ALCOHOLS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CALCIUM ISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ESTERS
EVEN-ODD NUCLEI
HALIDES
HALOGEN COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INOSITOLS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NEOPLASMS
NUCLEI
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HORMONES
RADIOISOTOPES
SACCHARIDES
SARCOMAS
SKELETAL DISEASES
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques