Arginine vasopressin promotes growth of rat glomerular mesangial cells in culture

Journal Article · Tue Nov 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6291514

Ganz, M B; Pekar, S K; Perfetto, M C; Sterzel, R B ^[1]

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1. Yale Univ. School of Medicine, New Haven, CT (USA)

Arginine vasopressin (AVP) binds specifically to vascular smooth muscle-like mesangial cells (MCs) and affects contraction. The authors tested whether this peptide also modulates growth behavior of rat MCs in early subculture (passage 2-5). Subconfluent, serum-starved MCs were exposed to AVP in the presence or absence of insulin. To assess DNA replication, MC uptake of ({sup 3}H)thymidine was determined on days 1, 2, and 3. AVP alone averaged a 1.97-fold increase in DNA synthesis at 24 h, whereas the mean stimulatory effects of AVP at 48 and 72 h were 7.21- and 5.42-fold, respectively. MCs exposed simultaneously to AVP and insulin showed potentiation of the mitogenic response to AVP alone. The V{sub 1}-receptor antagonist (1-({beta}-mercapto-{beta},{beta}-cyclopentamethylene proprionic acid), 2-(O-methyl-Tyr)-Arg)vasopressin (PMP) inhibited only AVP-induced promotion of MC growth. The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA) acutely stimulated MC proliferation but did not add to the AVP effect. They conclude that (1) AVP is strongly and specifically mitogenic for rat MCs, (2) the proliferative effect of AVP is mediated by TPA-like activation of protein kinase C, and (3) a maximal rise of Ca{sub i} need not be essential for the induction of AVP-elicited mitogenesis of MCs.

• OSTI ID: 6291514
• Journal Information: American Journal of Physiology; (USA), Vol. 255:5; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUSCLES
DNA REPLICATION
TRITIUM COMPOUNDS
UPTAKE
VASOPRESSIN
BIOCHEMISTRY
ARGININE
CELL CULTURES
PHOSPHOTRANSFERASES
RATS
RECEPTORS
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
CHEMISTRY
ENZYMES
HORMONES
HYDROGEN COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PITUITARY HORMONES
PROTEINS
RODENTS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques