Culture of ciliated and nonciliated cells from rat ductuli efferentes
The isolation and culture of ciliated and nonciliated cells from rat ductuli efferentes is described. Fragments of epithelium obtained after two collagenase digestions attached to plastic and to extracellular matrix and could be maintained in culture for at least 2 weeks. Ciliary beating in cells grown on epididymal extracellular matrix-coated plastic could be observed for up to 7 days in culture. Although cells maintained on this substrate retained organelles characteristic of cells in vivo, they assumed a flattened, squamous appearance. In contrast, cells growing on the surface of permeable supports impregnated with extracellular matrix were polarized and exhibited a cuboidal/columnar appearance. Androgen binding protein conjugated to colloidal gold was taken up by these cells via coated pits and was found sequentially in uncoated endosomes, multivesicular bodies and lysosomes.
- Research Organization:
- Georgetown Univ., Washington, DC
- OSTI ID:
- 6290895
- Journal Information:
- J. Androl.; (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EPITHELIUM
CYTOLOGICAL TECHNIQUES
ANDROGENS
CELL CONSTITUENTS
CELL CULTURES
COLLOIDS
GOLD ISOTOPES
LYSOSOMES
PROTEINS
RATS
SUBSTRATES
TESTES
ANDROSTANES
ANIMAL TISSUES
ANIMALS
BODY
DISPERSIONS
GONADS
HORMONES
ISOTOPES
MALE GENITALS
MAMMALS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RODENTS
STEROID HORMONES
STEROIDS
TISSUES
VERTEBRATES
550301* - Cytology- Tracer Techniques