Purification of the pyrazole-inducible cytochrome P-450 isozyme
The alcohol dehydrogenase inhibitor, pyrazole, appears to induce a cytochrome P-450 isozyme with properties similar to the ethanol-inducible P-450. The pyrazole-inducible P-450 isozyme was purified from the liver microsomes of rats treated with pyrazole essentially by the procedure of Ryan et al and also by chromatofocussing. The final preparation appeared homogenous by SDS-PAGE with an apparent molecular weight of 52,000, had a specific content of 11 nmoles P-450 per mg protein, showed very high activity of low K/sub m/ dimethylnitrosamine demethylase and produced a type II binding spectrum with dimethylsulfoxide. The enzyme was also active with aniline and aminopyrine as substrates. Pyrazole itself served as an excellent substrate with 4-hydroxy pyrazole being the product. An antibody against the pyrazole-inducible P-450 raised in chickens recognized a protein with mol.wt of about 52,000 in control microsomes. This band was highly enriched in microsomes from rats treated with pyrazole, 4-methyl-pyrazole, ethanol or acetone, but not phenobarbital or 3-methylcholanthrene. In summary, the pyrazole-inducible P-450 has been purified and appears to be identical in its catalytic and immunological properties to the alcohol-inducible P-450.
- Research Organization:
- Mount Sinai School of Medicine, New York, NY
- OSTI ID:
- 6290676
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
Purification and characterization of the dog hepatic cytochrome P-450 isozyme(s) responsible for the metabolism of 2,2',4,4',5,5'-hexachlorobiphenyl
Selective inhibition by chloramphenicol of pregnenolone-16. cap alpha. -carbonitrile-inducible rat liver cytochrome P-450 isozymes
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
3-METHYLCHOLANTHRENE
BIOLOGICAL EFFECTS
ACETONE
ETHANOL
MIXED-FUNCTION OXIDASES
BIOCHEMICAL REACTION KINETICS
FRACTIONATION
PYRAZOLES
DMSO
ELECTROPHORESIS
LIVER
MICROSOMES
MOLECULAR WEIGHT
PHENOBARBITAL
RATS
ALCOHOLS
ANESTHETICS
ANIMALS
ANTICONVULSANTS
AROMATICS
AZINES
AZOLES
BARBITURATES
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DRUGS
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
HYPNOTICS AND SEDATIVES
KETONES
KINETICS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIMIDINES
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SULFOXIDES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry