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Title: Cytokinetic and morphological changes in the lungs and lung-associated lymph nodes of rats after inhalation of fly ash

Abstract

Fischer-344 rats (male and female) were exposed to 36 mg/mT of fluidized bed coal combustion fly ash or sham-exposed for 7 hr/day, 5 days/week for 4 weeks, and sacrificed after 2 or 4 weeks of exposure and at 2, 22, and 42 weeks after the end of exposure. Animals were injected with tritiated thymidine 2 hr before sacrifice and autoradiographs prepared from 1- m sections of lung and lymph node tissue embedded in glycol methacrylate plastic. Differences in labeling indices of pulmonary epithelial cells, alveolar macrophages, airway epithelial cells, and cells of the lung-associated lymph nodes between the exposed and control animals were maximal after 2 and 4 weeks of exposure. Labeling indices for lung epithelial cells were about the same in control and exposed animals at 2, 22, and 42 weeks after the end of exposure. However, these values were elevated relative to earlier control levels. In contrast, morphological changes in the fly ash-exposed animals were most prominent after the end of the exposure. These changes included thickening of the alveolar walls, clusters of particle-filled macrophages in the alveolar region, and perivascular inflammation. Additionally, there were small granulomas in the alveolar region at 42 weeks after the end ofmore » exposure. Granulomas were also formed in the lung-associated lymph nodes and bronchus-associated lymphoid tissue. The authors conclude that the inhalation of fly ash alone had little detrimental effect upon the rat lung. However, the increases in proliferation indicate the potential for fly ash combined with a carcinogen to enhance the carcinogen's effect. 50 references, 10 figures.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Inhalation Toxicology Research Institute, Albuquerque, NM
OSTI Identifier:
6289822
DOE Contract Number:  
AC04-76EV01013
Resource Type:
Conference
Journal Name:
Environ. Res.; (United States)
Additional Journal Information:
Journal Volume: 35; Conference: Annual meeting of the Society of Toxicology, Las Vegas, NV, USA, 8-11 Mar 1983
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; FLY ASH; INHALATION; LUNGS; GRANULOMAS; MORPHOLOGICAL CHANGES; LYMPH NODES; AUTORADIOGRAPHY; CARCINOGENS; EPITHELIUM; EXPERIMENTAL DATA; MACROPHAGES; RATS; SYNERGISM; THYMIDINE; TOXICITY; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AEROSOL WASTES; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; ASHES; AZINES; BODY; CONNECTIVE TISSUE CELLS; DATA; DISEASES; HETEROCYCLIC COMPOUNDS; INFORMATION; INTAKE; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LYMPHATIC SYSTEM; MAMMALS; NEOPLASMS; NUCLEOSIDES; NUCLEOTIDES; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHAGOCYTES; PYRIMIDINES; RESIDUES; RESPIRATORY SYSTEM; RIBOSIDES; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; WASTES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 550201 - Biochemistry- Tracer Techniques

Citation Formats

Shami, S G, Silbaugh, S A, Hahn, F F, Griffith, W C, and Hobbs, C H. Cytokinetic and morphological changes in the lungs and lung-associated lymph nodes of rats after inhalation of fly ash. United States: N. p., 1984. Web.
Shami, S G, Silbaugh, S A, Hahn, F F, Griffith, W C, & Hobbs, C H. Cytokinetic and morphological changes in the lungs and lung-associated lymph nodes of rats after inhalation of fly ash. United States.
Shami, S G, Silbaugh, S A, Hahn, F F, Griffith, W C, and Hobbs, C H. 1984. "Cytokinetic and morphological changes in the lungs and lung-associated lymph nodes of rats after inhalation of fly ash". United States.
@article{osti_6289822,
title = {Cytokinetic and morphological changes in the lungs and lung-associated lymph nodes of rats after inhalation of fly ash},
author = {Shami, S G and Silbaugh, S A and Hahn, F F and Griffith, W C and Hobbs, C H},
abstractNote = {Fischer-344 rats (male and female) were exposed to 36 mg/mT of fluidized bed coal combustion fly ash or sham-exposed for 7 hr/day, 5 days/week for 4 weeks, and sacrificed after 2 or 4 weeks of exposure and at 2, 22, and 42 weeks after the end of exposure. Animals were injected with tritiated thymidine 2 hr before sacrifice and autoradiographs prepared from 1- m sections of lung and lymph node tissue embedded in glycol methacrylate plastic. Differences in labeling indices of pulmonary epithelial cells, alveolar macrophages, airway epithelial cells, and cells of the lung-associated lymph nodes between the exposed and control animals were maximal after 2 and 4 weeks of exposure. Labeling indices for lung epithelial cells were about the same in control and exposed animals at 2, 22, and 42 weeks after the end of exposure. However, these values were elevated relative to earlier control levels. In contrast, morphological changes in the fly ash-exposed animals were most prominent after the end of the exposure. These changes included thickening of the alveolar walls, clusters of particle-filled macrophages in the alveolar region, and perivascular inflammation. Additionally, there were small granulomas in the alveolar region at 42 weeks after the end of exposure. Granulomas were also formed in the lung-associated lymph nodes and bronchus-associated lymphoid tissue. The authors conclude that the inhalation of fly ash alone had little detrimental effect upon the rat lung. However, the increases in proliferation indicate the potential for fly ash combined with a carcinogen to enhance the carcinogen's effect. 50 references, 10 figures.},
doi = {},
url = {https://www.osti.gov/biblio/6289822}, journal = {Environ. Res.; (United States)},
number = ,
volume = 35,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1984},
month = {Sun Jan 01 00:00:00 EST 1984}
}

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