Application of denaturing gradient gel electrophoresis to detect DNA sequence differences encoding apolipoprotein E isoforms

Journal Article · Thu Apr 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1166· OSTI ID:6287903

Parker, S; Angelico, M C; Laffel, L; Krolewski, A S ^[1]

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1. Joslin Diabetes Center, Boston, MA (United States) Harvard Medical School, Boston, MA (United States)

Apolipoprotein E (apoE) plays an important role in plasma lipid metabolism. Three common isoforms of this protein have been identified by the isoelectric focusing method. In this report the authors describe a new method for distinguishing these isoforms. Their method employs PCR amplification of the DNA sequence of exon 4 in the apoE gene followed by denaturing gradient gel electrophoresis (DGGE) to distinguish its different melting characteristics. Identification of the ApoE isoforms through DNA melting behavior rather than protein charge differences eliminates the problems associated with isoelectric focusing and facilitates screening for additional mutations at the apoE locus. 12 refs., 2 figs.

• OSTI ID: 6287903
• Journal Information: Genomics; (United States), Vol. 16:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
APOLIPOPROTEINS
ISOMERS
DNA
AMPLIFICATION
ELECTROPHORESIS
DETECTION
DNA SEQUENCING
LIPIDS
LIPOPROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics