Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA

Branch, A D; Benenfeld, B J; Robertson, H D

doi:10.1073/pnas.82.19.6590

Title: Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA

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Abstract

The positions of intramolecular crosslinks induced by irradiation with ultraviolet light were mapped into potato spindle tuber viroid RNA and HeLa 5S rRNA. Crosslinking in each of these molecules occurred at a single major site, which was located by RNA fingerprinting and secondary analysis. Various lines of evidence suggest that these crosslinks identify a previously undescribed element of local tertiary structure common to these two widely divergent RNA molecules: (i) both crosslinks occur in an identical eight-base context, with the sequence 5 GGGAA 3 on one side and the sequence 5 UAC 3 on the other; (ii) both crosslinks connect bases that are not thought to be involved in conventional hydrogen bonding, within regions usually depicted as single-stranded loops flanked by short helical segments; and (iii) both crosslinks connect a purine and a pyrimidine residue, and both may generate the same G-U dimer. Furthermore, it is likely that the crosslinking site is of functional significance because it is located within the most highly conserved region of the viroid sequence and involves bases that are essentially invariant among eukaryotic 5S rRNA molecules.

Authors:: Branch, A D; Benenfeld, B J; Robertson, H D

Publication Date:: Tue Oct 01 00:00:00 EDT 1985

Research Org.:: Rockefeller Univ., New York, NY

OSTI Identifier:: 6287244

Resource Type:: Journal Article

Journal Name:: Proc. Natl. Acad. Sci. U.S.A.; (United States)

Additional Journal Information:: Journal Volume: 19

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CROSS-LINKING; RADIOINDUCTION; RNA; BIOLOGICAL RADIATION EFFECTS; HELA CELLS; RIBOSOMES; ULTRAVIOLET RADIATION; VIRUSES; BIOLOGICAL EFFECTS; CELL CONSTITUENTS; CHEMICAL REACTIONS; ELECTROMAGNETIC RADIATION; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANOIDS; PARASITES; POLYMERIZATION; RADIATION EFFECTS; RADIATIONS; 560121* - Radiation Effects on Cells- External Source- (-1987)

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                    Branch, A D, Benenfeld, B J, and Robertson, H D. Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA.  United States: N. p., 1985. 
        Web.  doi:10.1073/pnas.82.19.6590.

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                    Branch, A D, Benenfeld, B J, & Robertson, H D. Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA.  United States.  https://doi.org/10.1073/pnas.82.19.6590

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                    Branch, A D, Benenfeld, B J, and Robertson, H D. 1985.  
        "Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA".  United States.  https://doi.org/10.1073/pnas.82.19.6590.

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@article{osti_6287244,

  title        = {Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA},

  author       = {Branch, A D and Benenfeld, B J and Robertson, H D},

  abstractNote = {The positions of intramolecular crosslinks induced by irradiation with ultraviolet light were mapped into potato spindle tuber viroid RNA and HeLa 5S rRNA. Crosslinking in each of these molecules occurred at a single major site, which was located by RNA fingerprinting and secondary analysis. Various lines of evidence suggest that these crosslinks identify a previously undescribed element of local tertiary structure common to these two widely divergent RNA molecules: (i) both crosslinks occur in an identical eight-base context, with the sequence 5 GGGAA 3 on one side and the sequence 5 UAC 3 on the other; (ii) both crosslinks connect bases that are not thought to be involved in conventional hydrogen bonding, within regions usually depicted as single-stranded loops flanked by short helical segments; and (iii) both crosslinks connect a purine and a pyrimidine residue, and both may generate the same G-U dimer. Furthermore, it is likely that the crosslinking site is of functional significance because it is located within the most highly conserved region of the viroid sequence and involves bases that are essentially invariant among eukaryotic 5S rRNA molecules.},

  doi          = {10.1073/pnas.82.19.6590},

  url          = {https://www.osti.gov/biblio/6287244},
  journal      = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number       = ,

  volume       = 19,

  place        = {United States},

  year         = {Tue Oct 01 00:00:00 EDT 1985},

  month        = {Tue Oct 01 00:00:00 EDT 1985}

}

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