Asbestos-associated chromosomal changes in human mesothelial cells

Journal Article · Sat Jun 01 00:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.11.3884· OSTI ID:6285955

Lechner, J F; Tokiwa, T; LaVeck, M; Benedict, W F; Banks-Schlegel, S; Yeager, H Jr; Banerjee, A; Harris, C C

Replicative cultures of human pleural mesothelial cells were established from noncancerous adult donors. The cells exhibited normal mesothelial cell characteristics including keratin, hyaluronic acid mucin, and long branched microvilli, and they retained the normal human karyotype until senescence. The mesothelial cells were 10 and 100 times more sensitive to the cytotoxic effects of asbestos fibers than normal human bronchial epithelial or fibroblastic cells, respectively. In addition, cultures of mesothelial cells that survived two cytotoxic exposures of amosite fibers were aneuploid with consistent specific chromosomal losses indicative of clonal origin. These aneuploid cells exhibit both altered growth control properties and a population doubling potential of >50 divisions beyond the culture life span (30 doublings) of the control cells.

• Research Organization: National Institutes of Health (NIH), Bethesda, MD (United States)
• OSTI ID: 6285955
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:11
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ASBESTOS
GENETIC EFFECTS
RESPIRATORY TRACT CELLS
ANEUPLOIDY
CARCINOGENESIS
CELL CULTURES
LUNGS
MAN
SCANNING ELECTRON MICROSCOPY
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
ELECTRON MICROSCOPY
MAMMALS
MICROSCOPY
ORGANS
PATHOGENESIS
PLOIDY
PRIMATES
RESPIRATORY SYSTEM
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)