In vitro growth inhibition of mastitis causing bacteria by phenolics and metal chelators

Journal Article · Fri Nov 01 00:00:00 EST 1985 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(85)81199-1· OSTI ID:6285852

Chew, B P; Tjoelker, L W; Tanaka, T S

Antimicrobial activities of three phenolic compounds and four metal chelators were tested at 0, 250, 500, and 1000 ppm in vitro against four major mastitis-causing bacteria, Streptococcus agalactiae, Staphylococcus aureus, Klebsiella pnuemoniae, and Escherichia coli. Overall, butylated hydroxyanisole and tert-butylhydroquinone showed the greatest antimicrobial activity. These phenolics were bactericidal at 250 to 500 ppm against all four bacteria tested. The butylated hydroxytoluene was bactericidal against the gram-positive bacteria but was ineffective against the coliforms. At 250 ppm, disodium ethylenediaminetetraacetic acid was bactericidal against the gram-positive bacteria but much less effective against the gram-negatives. However, diethylene-triaminepentaacetic acid was more growth inhibitory than ethylenediaminetetraacetic acid against the gram-negative bacteria and especially against Escherichia coli. All other compounds were generally much less effective or ineffective against all four microorganisms. Therefore, butylated hydroxyanisole, butylated hydroxytoluene, tert-butylhydroquinone, ethylenediaminetetraacetic acid, and diethylenetriaminepentaacetic acid may have practical implications in the prevention or treatment of bovine mastitis.

• Research Organization: Washington State Univ., Pullman, WA (United States)
• OSTI ID: 6285852
• Journal Information: J. Dairy Sci.; (United States), Vol. 68:11
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIOXIDANTS
BIOLOGICAL EFFECTS
BACTERIA
GROWTH
EDTA
CATTLE
COMPARATIVE EVALUATIONS
ESCHERICHIA COLI
IN VITRO
INFECTIOUS DISEASES
INHIBITION
KLEBSIELLA
MAMMARY GLANDS
PHENOLS
STAPHYLOCOCCUS
STREPTOCOCCUS
AMINO ACIDS
ANIMALS
AROMATICS
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
DISEASES
DOMESTIC ANIMALS
GLANDS
HYDROXY COMPOUNDS
MAMMALS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RUMINANTS
VERTEBRATES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)