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Title: Atrial natriuretic factor in maternal and fetal sheep

Abstract

To determine atrial natriuretic factor (ANF) concentrations in the circulation and body fluids of adult pregnant sheep and their fetuses, pregnant ewes were anesthetized with pentobarbital sodium, and the fetuses were exteriorized for sampling. ANF concentration, as measured by radioimmunoassay, was 47 +/- 6 (SE) pg/ml in maternal plasma, which was significantly higher than the 15 +/- 3 pg/ml in maternal urine. In the fetus, plasma ANF concentration was 265 +/- 49 pg/ml, 5.6 times that in maternal plasma. No umbilical arterial and venous difference in ANF concentration was observed. Fetal urine ANF concentration was significantly lower than that in fetal plasma, and was similar to that measured in amniotic and allantoic fluid. In chronically catheterized maternal and fetal sheep, fetal plasma ANF was again 5.1 times that in maternal plasma, and these levels were not different from those measured in acutely anesthetized animals. These results demonstrate that immunoreactive ANF is present in the fetal circulation at levels higher than those found in the mother. The low concentration of ANF in fetal urine suggests that ANF is probably metabolized and/or reabsorbed by the fetal kidney.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of California, San Diego, La Jolla
OSTI Identifier:
6283811
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 252:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; DIURETICS; RADIOIMMUNOASSAY; ANESTHESIA; BIOLOGICAL STRESS; BLOOD CIRCULATION; BLOOD PLASMA; FEMALES; FETUSES; PREGNANCY; SHEEP; URINE; ANIMALS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BLOOD; BODY FLUIDS; DOMESTIC ANIMALS; DRUGS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; MAMMALS; MATERIALS; RADIOASSAY; RADIOIMMUNOLOGY; RUMINANTS; TRACER TECHNIQUES; VERTEBRATES; WASTES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Cheung, C Y, Gibbs, D M, and Brace, R A. Atrial natriuretic factor in maternal and fetal sheep. United States: N. p., 1987. Web.
Cheung, C Y, Gibbs, D M, & Brace, R A. Atrial natriuretic factor in maternal and fetal sheep. United States.
Cheung, C Y, Gibbs, D M, and Brace, R A. 1987. "Atrial natriuretic factor in maternal and fetal sheep". United States.
@article{osti_6283811,
title = {Atrial natriuretic factor in maternal and fetal sheep},
author = {Cheung, C Y and Gibbs, D M and Brace, R A},
abstractNote = {To determine atrial natriuretic factor (ANF) concentrations in the circulation and body fluids of adult pregnant sheep and their fetuses, pregnant ewes were anesthetized with pentobarbital sodium, and the fetuses were exteriorized for sampling. ANF concentration, as measured by radioimmunoassay, was 47 +/- 6 (SE) pg/ml in maternal plasma, which was significantly higher than the 15 +/- 3 pg/ml in maternal urine. In the fetus, plasma ANF concentration was 265 +/- 49 pg/ml, 5.6 times that in maternal plasma. No umbilical arterial and venous difference in ANF concentration was observed. Fetal urine ANF concentration was significantly lower than that in fetal plasma, and was similar to that measured in amniotic and allantoic fluid. In chronically catheterized maternal and fetal sheep, fetal plasma ANF was again 5.1 times that in maternal plasma, and these levels were not different from those measured in acutely anesthetized animals. These results demonstrate that immunoreactive ANF is present in the fetal circulation at levels higher than those found in the mother. The low concentration of ANF in fetal urine suggests that ANF is probably metabolized and/or reabsorbed by the fetal kidney.},
doi = {},
url = {https://www.osti.gov/biblio/6283811}, journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 252:2,
place = {United States},
year = {Sun Feb 01 00:00:00 EST 1987},
month = {Sun Feb 01 00:00:00 EST 1987}
}