A radiation hybrid map of the distal short arm of human chromosome II, containing the Beckwith-Weidemann and associated embroyonal tumor disease loci
- Univ. of California, San Francisco (United States)
- Univ. of Michigan, Ann Arbor (United States)
- National Cancer Institute, Bethesda (United States)
The authors describe a high-resolution radiation hybrid (RH) map of the distal short arm of human chromosome 11 containing the Beckwith-Weidemann gene and the associated embryonal tumor disease loci. Thirteen human 11p15 genes and 17 new anonymous probes were mapped by a statistical analysis of the cosegregation of markers in 102 rodent-human radiation hybrids retaining fragments of human chromosome 11. The 17 anonymous probes were generated from lambda phage containing human 11p15.5 inserts, by using ALU-PCR. A comprehensive map of all 30 loci and a framework map of nine clusters of loci ordered at odds of 1,000:1 were constructed by a multipoint maximum-likelihood approach by using the computer program RHMAP. This RH map localizes one new gene to chromosome 11p15 (WEE1), provides more precise order information for several 11p15 genes (CTSD, H19, HPX,.ST5, RNH, and SMPD1), confirms previous map orders for other 11p15 genes (CALCA, PTH, HBBC, TH, HRAS, and DRD4), and maps 17 new anonymous probes within the 11p15.5 region. This RH map should prove useful in better defining the positions of the Beckwith-Weidemann and associated embryonal tumor disease-gene loci. 41 refs., 1 fig., 2 tabs.
- OSTI ID:
- 6281686
- Journal Information:
- American Journal of Human Genetics; (United States), Vol. 52:5; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CONGENITAL DISEASES
GENES
HUMAN CHROMOSOMES
MAPPING
ANIMAL GROWTH
CONGENITAL MALFORMATIONS
DNA HYBRIDIZATION
EMBRYOS
NEOPLASMS
PROGRAMMING LANGUAGES
TONGUE
BODY
CHROMOSOMES
DIGESTIVE SYSTEM
DISEASES
GROWTH
HYBRIDIZATION
MALFORMATIONS
ORAL CAVITY
ORGANS
PATHOLOGICAL CHANGES
550400* - Genetics