Effect of the oxygen transfer rate (OSR) on the formation of cellulases by Trichoderma viride in submersion culture
The formation of cellulases by Trichoderma viride in a medium containing cellulose as a sole source of carbon depends on the oxygen transfer rate (OSR); the OSR, on the other hand, depends on the concentration of cellulose in the medium because the concentration of cellulose strongly affects the viscosity of the medium. In the work presented here, the dependence has been determined for the oxygen transfer rate on geometric relations and viscosity in cellulose-containing media during cultivation in shaken flasks, and the oxygen transfer rates on NRE, NG, and Na during cultivation in a laboratory fermentor of 3000-mL volume. Two cellulosic materials have been compared with a different effect on viscosity; microcrystalline beach cellulose and fibrous cellulose. It has been found that, in an applicable range of concentration, microcrystalline cellulose does not affect the oxygen transfer rate (at concentrations up to 3%). Fibrous cellulose increases the OSR during cultivation in shake flasks but decreases its during cultivation in fermentors. On the basis of these results, the optimizing has been carried out on the cultivation conditions in fermentors. (Refs. 50).
- Research Organization:
- Research Inst of Food Industry, Na belidle 21, 150 38 Prague 5, Czechoslovakia
- OSTI ID:
- 6281461
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 24:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
BIOSYNTHESIS
CELLULOSE
OPTIMIZATION
CULTURE MEDIA
ENZYMATIC HYDROLYSIS
OXYGEN
TRICHODERMA VIRIDE
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ELEMENTS
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
NONMETALS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550700 - Microbiology