Posttransfusion survival of red cells frozen for 8 weeks after 42-day liquid storage in AS-3

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Transfusion (Philadelphia); (United States)

DOI:https://doi.org/10.1046/j.1537-2995.1989.29389162725.x· OSTI ID:6279587

Rathbun, E J; Nelson, E J; Davey, R J

Current standards recommend that red cells (RBCs) should be frozen within 6 days of donation. There are situations, however, in which it is desirable to freeze RBCs that are older than 6 days, such as for the salvage of rare or autologous units. To determine the therapeutic efficacy of RBCs frozen after prolonged liquid storage, standard units were drawn from nine normal donors and stored at 4 degrees C for 42 days in a nutrient-additive solution, AS-3. 51CrRBC survival assays were performed (24-hour survival: 78.2 +/- 12.4%; n = 8) and the units were frozen at -80 degrees C in glycerol for 8 weeks. After deglycerolization, the mean RBC recovery was 81.0 +/- 4.1 percent and the mean 24-hour 51Cr survival was 78.0 +/- 9.1 percent. The index of therapeutic effectiveness (ITE) was determined by multiplying the postdeglycerolization 24-hour 51Cr survival by the mean RBC recovery (63.3 +/- 9.2). ITE values greater than 60 percent (75% 51Cr survival x 80% RBC recovery) were considered acceptable. Mean adenosine triphosphate levels declined from an initial 3.81 +/- 0.56 micromol per g of hemoglobin to 2.33 +/- 0.55 micromol per g after frozen storage. These findings show that an acceptable percentage of RBCs survives frozen storage after maximum liquid storage (mean ITE greater than 60%). If necessary, RBCs stored in AS-3 can be frozen at any time before 42 days.

• Research Organization: Warren G. Magnuson Clinical Center, Bethesda, MD (USA)
• OSTI ID: 6279587
• Journal Information: Transfusion (Philadelphia); (United States), Vol. 29:3
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ERYTHROCYTES
SURVIVAL CURVES
ATP
BIOLOGICAL RECOVERY
CHROMIUM 51
CITRATES
FREEZING
GLUCOSE
HEMOLYSIS
RADIOASSAY
STORAGE
TRACER TECHNIQUES
TRANSFUSIONS
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BETA DECAY RADIOISOTOPES
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CARBOXYLIC ACID SALTS
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEXOSES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LYSIS
MATERIALS
MONOSACCHARIDES
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
RADIOISOTOPES
RECOVERY
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550301* - Cytology- Tracer Techniques