(Anaerobic metabolism of aromatic compounds by phototrophic bacteria: biochemical aspects): Annual progress report, April 1988--March 1989

Technical Report · Sun Jan 01 00:00:00 EST 1989

DOI:https://doi.org/10.2172/6279514· OSTI ID:6279514

Harwood, C. S.; Gibson, J.

Intensive efforts to define a protocol for generating transposon mutations in R. palustris have continued. An indirect approach, in which a transposon is introduced into a pLAFR1 cosmid containing an approximately 20 kb fragment of R. palustris DNA from a lambda derivative containing Tn5, appears promising. Both plasmids can be introduced into E. coli, and the antibiotic resistances coded for by each subsequently mated into wild-type R. palustris. A mutant isolated following chemical mutagenesis which is unable to grow aerobically on 4-OH benzoate (CGA033) has been complemented biochemically by the same clone which functions in the absence of the transposon; in addition, and more importantly, this indirect tranposon mutagenesis appears to yield mutants with novel phenotypes affecting the anaerobic pathway for 4-OH benzoate utilization. 2 refs.

• Research Organization: Cornell Univ., Ithaca, NY (United States)
• DOE Contract Number: FG02-86ER13495
• OSTI ID: 6279514
• Report Number(s): DOE/ER/13495-T2; ON: DE89010644
• Resource Relation: Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
BENZOIC ACID
ANAEROBIC DIGESTION
PHENOLS
CATABOLISM
PROGRESS REPORT
RHODOPSEUDOMONAS
AROMATICS
BACTERIA
BIOCONVERSION
CARBOXYLIC ACIDS
DIGESTION
DOCUMENT TYPES
HYDROXY COMPOUNDS
MANAGEMENT
METABOLISM
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROCESSING
WASTE MANAGEMENT
WASTE PROCESSING
550500* - Metabolism
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)