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Title: Further studies on the effect of nitrogen dioxide on mast cells: The effect of the metabolite, nitrite

Abstract

To evaluate the relationship between atmospheric nitrogen dioxide exposure and the development of allergic diseases, the effects of nitrite as a chemical product of inhaled nitrogen dioxide on mast cell functions were investigated. We have studied nitride-induced histamine release from two functionally distinct mast cell populations, namely peritoneal mast cells (PMC) and intestinal mucosal mast cells (IMMC) of Nippostrongylus brasiliensis-infected rats. High concentrations of nitrite alone (10, 20, and 50 mM) induced histamine release from IMMC, but not from PMC. Moreover, histamine release from PMC and IMMC stimulated with sensitizing antigen was significantly enhanced by pretreatment with 50 mM nitrite or nitrate. No differences in histamine release from nitrite-treated and control PMC were seen below 1 mM. To investigate the effect of nitrite on tumor cell cytotoxic activity, PMC were incubated with various concentrations of nitrite. Pretreatment with 5 and 50 mM nitrite markedly depressed tumor necrosis factor (TNF)-[alpha]-dependent natural cytotoxicity of PMC for the tumor target WEHI-164. Thus, high concentrations of nitrite enhanced mast cell histamine release, but depressed TNF-[alpha]-dependent cytotoxicity. However, low concentrations of nitrite (<1 mM) that would normally be produced by short-term atmospheric exposure to nitrogen dioxide may have no significant effects on mast cell functions.more » 27 refs., 3 figs., 1 tab.« less

Authors:
 [1];  [2]; ;  [3]
  1. National Institute for Environmental Studies, Onogawa (Japan)
  2. The Jikei Univ. School of Medicine, Tokyo (Japan)
  3. Univ. of Calgary, Alberta (Canada)
Publication Date:
OSTI Identifier:
6279480
Resource Type:
Journal Article
Journal Name:
Environmental Research; (United States)
Additional Journal Information:
Journal Volume: 61:2; Journal ID: ISSN 0013-9351
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; AIR POLLUTION; BIOLOGICAL EFFECTS; ALLERGY; ETIOLOGY; MAST CELLS; DYNAMIC FUNCTION STUDIES; NITRITES; BIOLOGICAL ACCUMULATION; HISTAMINE; NECROSIS; NIPPOSTRONGYLUS; NITROGEN DIOXIDE; RATS; AMINES; ANIMAL CELLS; ANIMALS; ASCHELMINTHES; AZOLES; CHALCOGENIDES; CONNECTIVE TISSUE CELLS; HELMINTHS; HETEROCYCLIC COMPOUNDS; HOOKWORM; IMIDAZOLES; MAMMALS; NEMATODES; NITROGEN COMPOUNDS; NITROGEN OXIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PARASITES; PATHOLOGICAL CHANGES; POLLUTION; RODENTS; SOMATIC CELLS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550200 - Biochemistry

Citation Formats

Fujimaki, Hidekazu, Ozawa, Masashi, Bissonnette, E, and Befus, A D. Further studies on the effect of nitrogen dioxide on mast cells: The effect of the metabolite, nitrite. United States: N. p., 1993. Web. doi:10.1006/enrs.1993.1066.
Fujimaki, Hidekazu, Ozawa, Masashi, Bissonnette, E, & Befus, A D. Further studies on the effect of nitrogen dioxide on mast cells: The effect of the metabolite, nitrite. United States. https://doi.org/10.1006/enrs.1993.1066
Fujimaki, Hidekazu, Ozawa, Masashi, Bissonnette, E, and Befus, A D. 1993. "Further studies on the effect of nitrogen dioxide on mast cells: The effect of the metabolite, nitrite". United States. https://doi.org/10.1006/enrs.1993.1066.
@article{osti_6279480,
title = {Further studies on the effect of nitrogen dioxide on mast cells: The effect of the metabolite, nitrite},
author = {Fujimaki, Hidekazu and Ozawa, Masashi and Bissonnette, E and Befus, A D},
abstractNote = {To evaluate the relationship between atmospheric nitrogen dioxide exposure and the development of allergic diseases, the effects of nitrite as a chemical product of inhaled nitrogen dioxide on mast cell functions were investigated. We have studied nitride-induced histamine release from two functionally distinct mast cell populations, namely peritoneal mast cells (PMC) and intestinal mucosal mast cells (IMMC) of Nippostrongylus brasiliensis-infected rats. High concentrations of nitrite alone (10, 20, and 50 mM) induced histamine release from IMMC, but not from PMC. Moreover, histamine release from PMC and IMMC stimulated with sensitizing antigen was significantly enhanced by pretreatment with 50 mM nitrite or nitrate. No differences in histamine release from nitrite-treated and control PMC were seen below 1 mM. To investigate the effect of nitrite on tumor cell cytotoxic activity, PMC were incubated with various concentrations of nitrite. Pretreatment with 5 and 50 mM nitrite markedly depressed tumor necrosis factor (TNF)-[alpha]-dependent natural cytotoxicity of PMC for the tumor target WEHI-164. Thus, high concentrations of nitrite enhanced mast cell histamine release, but depressed TNF-[alpha]-dependent cytotoxicity. However, low concentrations of nitrite (<1 mM) that would normally be produced by short-term atmospheric exposure to nitrogen dioxide may have no significant effects on mast cell functions. 27 refs., 3 figs., 1 tab.},
doi = {10.1006/enrs.1993.1066},
url = {https://www.osti.gov/biblio/6279480}, journal = {Environmental Research; (United States)},
issn = {0013-9351},
number = ,
volume = 61:2,
place = {United States},
year = {Sat May 01 00:00:00 EDT 1993},
month = {Sat May 01 00:00:00 EDT 1993}
}