Existence of B/E and E receptors on Hep-G2 cells: a study using colloidal gold- and /sup 125/I-labeled lipoproteins
The presence of specific receptors for apolipoprotein B (low-density lipoproteins) and apolipoprotein E (HDL-E) on Hep-G2 cells and human skin fibroblasts was studied by chemical methods and by electron microscopy using a differential gold labeling technique. Fibroblasts bound both types of lipoproteins to one and the same receptor (B/E receptor) as deduced from competition experiments with HDL-E and LDL. Labeled HDL-E, on the other hand, was only partially displaced by cold LDL but was completely displaced by unlabeled HDL-E. Scatchard analysis of lipoprotein binding to Hep-G2 cells revealed an approx 10 times higher binding affinity of apoE-containing lipoproteins as compared to apoB-containing ones. No differences between apoE- or apoB-containing lipoproteins with respect to the morphology of cell binding and intracellular processing were observed. The results are compatible with the concept that Hep-G2 cells possess two kinds of receptors, one specific for apoB- and apoE-containing lipoproteins (B/E receptor) and another specific for apoE only. From these studies we conclude that Hep-G2 cells may serve as a suitable model for studying the lipoprotein metabolism in the liver.
- Research Organization:
- County Hospital, Kecskemet, Hungary
- OSTI ID:
- 6279148
- Journal Information:
- Exp. Mol. Pathol.; (United States), Vol. 46:3
- Country of Publication:
- United States
- Language:
- English
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APOLIPOPROTEINS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
BIOLOGICAL MODELS
COLLOIDS
FIBROBLASTS
GOLD
IODINE 125
LABELLED COMPOUNDS
LIVER
MAN
SKIN
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISPERSIONS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
LIPOPROTEINS
MAMMALS
MEMBRANE PROTEINS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SOMATIC CELLS
TRANSITION ELEMENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques