Color regulation in the archaebacterial phototaxis receptor phoborhodopsin (sensory rhodopsin II)

Journal Article · Tue Sep 11 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00488a038· OSTI ID:6278181

Takahashi, T; Yan, B; Mazur, P; Derguini, F; Nakanishi, K; Spudich, J L ^[1]

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1. Albert Einstein College of Medicine, Bronx, NY (USA)

Phoborhodopsin, a repellent phototaxis receptor in Halobacterium halobium, exhibits vibrational fine structure, a feature that has not been identified for any other rhodopsin pigment at physiological temperatures. This conclusion follows form analysis of the absorption properties of the pigment in H. halobium membranes containing native retinal and an array of retinal analogues. The absorption spectrum of the native pigment has a maximum at 487 nm with a pronounced shoulder at 460 nm; however, the bandwidth is that expected for a single retinylidene species. Gaussian band-shape simulation with a spacing corresponding to the vibrational frequencies of polyene stretching modes reproduces the structured absorption spectra of native pigment as well as of analogue phoborhodopsin. Absorption shifts produced by a series of dihydroretinal and other retinal analogues strongly indicate that the dominant factor regulating the color of the pigment is planarization of the retinal ring with respect to the polyene chain.

• OSTI ID: 6278181
• Journal Information: Biochemistry; (USA), Vol. 29:36; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RHODOPSIN
ABSORPTION SPECTRA
COLOR
MOLECULAR STRUCTURE
PHOTOSYNTHETIC BACTERIA
PHOTOSYNTHETIC MEMBRANES
RETINOIC ACID
SPECTROPHOTOMETRY
CARBOXYLIC ACID ESTERS
ESTERS
MEMBRANES
OPTICAL PROPERTIES
ORGANIC COMPOUNDS
ORGANOLEPTIC PROPERTIES
PHYSICAL PROPERTIES
PIGMENTS
PROTEINS
SPECTRA
550200* - Biochemistry