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Title: In vivo effects of T-2 mycotoxin on synthesis of proteins and DNA in rat tissues

Abstract

Rats were given an ip injection of T-2 mycotoxin (T-2), the T-2 metabolite, T-2 tetraol (tetraol), or cycloheximide. Serum, liver, heart, kidney, spleen, muscle, and intestine were collected at 3, 6, and 9 hr postinjection after a 2-hr pulse at each time with (14C)leucine and (3H)thymidine. Protein and DNA synthesis levels in rats were determined by dual-label counting of the acid-precipitable fraction of tissue homogenates. Rats given a lethal dose of T-2, tetraol, or cycloheximide died between 14 and 20 hr. Maximum inhibition of protein synthesis at the earliest time period was observed in additional rats given the same lethal dose of the three treatments and continued for the duration of the study (9 hr). With sublethal doses of T-2 or tetraol, the same early decrease in protein synthesis was observed but, in most of the tissues, recovery was seen with time. In the T-2-treated rats. DNA synthesis in the six tissues studied was also suppressed, although to a lesser degree. With sublethal doses, complete recovery of DNA synthesis took place in four of the six tissues by 9 hr after toxin exposure. The appearance of newly translated serum proteins did not occur in the animals treated with T-2 mycotoxinmore » or cycloheximide, as evidenced by total and PCA-soluble serum levels of labeled leucine. An increase in tissue-pool levels of free leucine and thymidine in response to T-2 mycotoxin was also noted. T-2 mycotoxin, its metabolite, T-2 tetraol, and cycloheximide cause a rapid inhibition of protein and DNA synthesis in all tissue types studied. These results are compared with the responses seen in in vitro studies.« less

Authors:
;  [1]
  1. United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD (USA)
Publication Date:
OSTI Identifier:
6275017
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology; (USA)
Additional Journal Information:
Journal Volume: 105:3; Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CYCLOHEXIMIDE; BIOLOGICAL EFFECTS; PROTEINS; BIOSYNTHESIS; TOXINS; AMINO ACIDS; CARBON 14 COMPOUNDS; DNA REPLICATION; DOSE-RESPONSE RELATIONSHIPS; IN VIVO; LEUCINE; METABOLITES; RATS; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTIGENS; AZINES; CARBOXYLIC ACIDS; DRUGS; FUNGICIDES; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PESTICIDES; PYRIMIDINES; RIBOSIDES; RODENTS; SYNTHESIS; TOXIC MATERIALS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Thompson, W L, and Wannemacher, Jr, R W. In vivo effects of T-2 mycotoxin on synthesis of proteins and DNA in rat tissues. United States: N. p., 1990. Web. doi:10.1016/0041-008X(90)90151-J.
Thompson, W L, & Wannemacher, Jr, R W. In vivo effects of T-2 mycotoxin on synthesis of proteins and DNA in rat tissues. United States. https://doi.org/10.1016/0041-008X(90)90151-J
Thompson, W L, and Wannemacher, Jr, R W. 1990. "In vivo effects of T-2 mycotoxin on synthesis of proteins and DNA in rat tissues". United States. https://doi.org/10.1016/0041-008X(90)90151-J.
@article{osti_6275017,
title = {In vivo effects of T-2 mycotoxin on synthesis of proteins and DNA in rat tissues},
author = {Thompson, W L and Wannemacher, Jr, R W},
abstractNote = {Rats were given an ip injection of T-2 mycotoxin (T-2), the T-2 metabolite, T-2 tetraol (tetraol), or cycloheximide. Serum, liver, heart, kidney, spleen, muscle, and intestine were collected at 3, 6, and 9 hr postinjection after a 2-hr pulse at each time with (14C)leucine and (3H)thymidine. Protein and DNA synthesis levels in rats were determined by dual-label counting of the acid-precipitable fraction of tissue homogenates. Rats given a lethal dose of T-2, tetraol, or cycloheximide died between 14 and 20 hr. Maximum inhibition of protein synthesis at the earliest time period was observed in additional rats given the same lethal dose of the three treatments and continued for the duration of the study (9 hr). With sublethal doses of T-2 or tetraol, the same early decrease in protein synthesis was observed but, in most of the tissues, recovery was seen with time. In the T-2-treated rats. DNA synthesis in the six tissues studied was also suppressed, although to a lesser degree. With sublethal doses, complete recovery of DNA synthesis took place in four of the six tissues by 9 hr after toxin exposure. The appearance of newly translated serum proteins did not occur in the animals treated with T-2 mycotoxin or cycloheximide, as evidenced by total and PCA-soluble serum levels of labeled leucine. An increase in tissue-pool levels of free leucine and thymidine in response to T-2 mycotoxin was also noted. T-2 mycotoxin, its metabolite, T-2 tetraol, and cycloheximide cause a rapid inhibition of protein and DNA synthesis in all tissue types studied. These results are compared with the responses seen in in vitro studies.},
doi = {10.1016/0041-008X(90)90151-J},
url = {https://www.osti.gov/biblio/6275017}, journal = {Toxicology and Applied Pharmacology; (USA)},
issn = {0041-008X},
number = ,
volume = 105:3,
place = {United States},
year = {Sat Sep 15 00:00:00 EDT 1990},
month = {Sat Sep 15 00:00:00 EDT 1990}
}