Comparison of several indium-111 ligands in labeling blood cells: effect of diethylpyrocarbonate and CO/sub 2/

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · J. Nucl. Med.; (United States)

OSTI ID:6274874

Goedemans, W T; de Jong, M M

The effect of oxine sulfate, oxine sulfonate, tropolone, and Merc (2 mercaptopyridine-1-oxide) were compared with oxine, with respect to their capability of labeling blood cells when complexed to indium-111 (/sup 111/In). Indium-111 oxine sulfate performed similarly to (/sup 111/In)oxine with regard to cell labeling capability. Indium-111 oxine sulfonate had no labeling ability. Indium-111 tropolone and Merc were not superior to (/sup 111/In)oxine as cell labeling agents. Carbon dioxide (CO/sub 2/) and a CO/sub 2/ generating compound, diethyl pyrocarbonate, dramatically improved the cell labeling ability in plasma of (/sup 111/In)tropolone and Merc. In the case of oxine, this improvement was less distinct. Theoretical aspects of the CO/sub 2/ cell labeling stimulating effect are discussed in terms of intra- and extracellular transferrin and lactoferrin iron (indium) binding capacity. Indium-111 tropolone behaved favorably with respect to inhibition of leukocyte migration, compared with oxine and Merc. Combined with the property of easy cell labeling and good solubility in water, also in the complexed state, tropolone must be regarded as the most suitable cell labeling ligand.

• Research Organization: B.V. Research and Development Department, Petten, The Netherlands
• OSTI ID: 6274874
• Journal Information: J. Nucl. Med.; (United States), Vol. 6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD CELLS
LABELLING
LIGANDS
COMPARATIVE EVALUATIONS
BLOOD PLATELETS
CARBON DIOXIDE
CARBONATES
CATTLE
ERYTHROCYTES
IN VITRO
INDIUM 111
LEUKOCYTES
ORGANOMETALLIC COMPOUNDS
OXINE
PYRIDINES
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
QUINOLINES
RADIOISOTOPES
RUMINANTS
VERTEBRATES
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