Vertical spectrophotometry for measuring antibodies to a variety of microbes using a microimmunoenzymatic assay on a flat surface
A novel micro-immunoenzymatic method was developed based on ELISA principles. It is performed with as little as 5 ..mu..l of each reagent on a flat, circular area a few mm in diameter. The reaction area is encircled by an extrathin layer of a hydrophobic material avoiding diffusion of reagents. These stay drop-shaped which allows OD readings directly in the drop of enzyme substrate added in the last step of the assay, using a small, low-cost, vertical beam spectrophotometer. Many reaction areas (18 or 30, 3 or 2 mm in diameter, respectively) can be printed on a standard histopathology glass slide. One operator can assay 100 samples per run in 2 hr, using 6 slides precoated with antigen. This slide micro-immunoenzymatic assay (micro-SIA) probed useful for measuring antibodies to a variety of eu- and archae-bacteria (cocci, rods, spirilla, sarcina), fungi, protozoa and viruses, in sera from several animal species and in medium from cultures of antibody-forming cells. Other important advantages of micro-SIA are its reproducibility, negligible background and simplicity. It can be performed in unsophisticated laboratories and it can be used for field studies, since it is amenable to a semi-quantitative evaluation by eye readings.
- Research Organization:
- NYSDH, Albany
- OSTI ID:
- 6272992
- Journal Information:
- Abstr. Annu. Meet. Am. Soc. Microbiol.; (United States), Vol. C161
- Country of Publication:
- United States
- Language:
- English
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