Calmodulin-binding proteins are developmentally regulated in gametes and embryos of fucoid algae

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Dev. Biol.; (United States)

DOI:https://doi.org/10.1016/S0012-1606(89)80004-1· OSTI ID:6272157

Brawley, S H; Roberts, D M

Calcium-binding proteins and calmodulin-binding proteins were identified in gametes and zygotes of the marine brown algae Fucus vesiculosus, Fucus distichus, and Pelvetia fastigiata using gel (SDS-PAGE) overlay techniques. A calcium current appears to be important during cell polarization in fucoid zygotes, but there are no biochemical data on calcium-binding proteins in these algae. By using a sensitive 45Ca2+ overlay method designed to detect high-affinity calcium-binding proteins, at least 9-11 polypeptides were detected in extracts of fucoid gametes and zygotes. All samples had calcium-binding proteins with apparent molecular weights of about 17 and 30 kDa. A 17-kDa calcium-binding protein was purified by calcium-dependent hydrophobic chromatography and was identified as calmodulin by immunological and enzyme activator criteria. A 125I-calmodulin overlay assay was used to identify potential targets of calmodulin action. Sperm contained one major calmodulin-binding protein of about 45 kDa. Eggs lacked major calmodulin-binding activity. A 72-kDa calmodulin-binding protein was prominent in zygotes from 1-65 hr postfertilization. Both calmodulin-binding proteins showed calcium-dependent binding activity. Overall, the data suggest that the appearance and distribution of certain calcium-binding and calmodulin-binding proteins are under developmental regulation, and may reflect the different roles of calcium during fertilization and early embryogenesis.

• Research Organization: Vanderbilt Univ., Nashville, TN (USA)
• OSTI ID: 6272157
• Journal Information: Dev. Biol.; (United States), Vol. 131:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALGAE
ONTOGENESIS
PROTEINS
TISSUE DISTRIBUTION
AUTORADIOGRAPHY
CALCIUM 45
CALMODULIN
CHROMATOGRAPHY
EGGS
ELECTROPHORESIS
EMBRYOS
FRACTIONATION
MOLECULAR WEIGHT
SPERMATOZOA
ALKALINE EARTH ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
EVEN-ODD NUCLEI
GAMETES
GERM CELLS
INTERMEDIATE MASS NUCLEI
ISOTOPES
NUCLEI
ORGANIC COMPOUNDS
PLANTS
RADIOISOTOPES
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques