Effects of radiofrequency radiation and simultaneous exposure with mitomycin C on the frequency of sister chromatid exchanges in Chinese hamster ovary cells
Chinese hamster ovary (CHO) cells were exposed for 2 hr with and without mitomycin C (MMC) (1 X 10(-8)M) to pulsed wave radiofrequency radiation (RFR) at 2450 MHz. The repetition rate of 25,000 pulses per sec (pps), pulse width of 10 microseconds, and exposure geometry used, resulted in a specific absorption rate (SAR) of 33.8 W/kg. The following exposure regimens were used: a 37 degrees C water bath control; a water bath temperature control (TC) in which the continuously monitored medium temperature closely followed the temperature rise in the RFR-exposed flasks; and the RFR-exposed cells in a water bath set at 37 degrees C prior to exposure. RFR exposure resulted in a maximum cell culture medium temperature of 39.2 degrees C. In the absence of MMC, there was no significant increase in sister chromatid exchange (SCE) in the RFR-exposed or TC groups over that of the 37 degrees C control. When a simultaneous treatment of RFR and MMC occurred there was no statistical difference in SCE frequency from that caused by chemical treatment alone.
- Research Organization:
- Univ. of Texas Health Science Center, San Antonio
- OSTI ID:
- 6271423
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
OVARIES
SISTER CHROMATID EXCHANGES
RADIOWAVE RADIATION
GENETIC EFFECTS
CHO CELLS
MITOMYCIN
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BODY
CHROMOSOMAL ABERRATIONS
DRUGS
ELECTROMAGNETIC RADIATION
FEMALE GENITALS
GONADS
MUTATIONS
ORGANS
RADIATIONS
560400* - Other Environmental Pollutant Effects