Method for the preparation of size marker for synthetic oligonucleotides

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90449-5· OSTI ID:6271417

Jing, G Z; Liu, A; Leung, W C

Terminal deoxynucleotidyltransferase was used for the addition of (..cap alpha..-/sup 32/P)dCTP to the 3'-OH termini of oligo(dT)/sub 12-18/. A collection of oligonucleotides with chain lengths ranging continuously from 13-mer to over 100-mer was generated. The reaction mixture was then mixed with oligo(dT)/sub 12-18/ labeled with (..gamma..-/sup 32/P)ATP by T/sub 4/ polynucleotide kinase. A sequence ladder with the bottom base as 12-mer was then formed. These oligonucleotides served as size marker for the purification and identification of oligonucleotides on polyacrylamide gel.

• Research Organization: Univ. of Alberta, Edmonton (Canada)
• OSTI ID: 6271417
• Journal Information: Anal. Biochem.; (United States), Vol. 155
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NUCLEIC ACIDS
BIOSYNTHESIS
NUCLEOTIDES
ATP
CREATINE
ELECTROPHORESIS
LABELLED COMPOUNDS
PHOSPHORUS 32
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques