Investigation of the pharmacokinetics and mechanism of the action of Eleutherococcus glycosides. VIII. Investigation of the activation of natural killers by Eleutherococcus extract
The authors studied whether there is activation of the cells of natural resistance in intact mice that have received Eleutherococcus extract (EE) as well as how EE influences the activity of the normal killers, proliferation of splenocytes of mice, and the synthesis of lymphokines (interferon, interleukin-2). The proliferation of splenocytes with concanavalin A was estimated according to the incorporation of (/sup 3/H)thymidine in a 96-well plate. Titration of interleukin-2 was performed on three-day concanavalin A partially in the presence of (/sup 3/H)thymidine. Splenocytes of intact mice were cultured for five days in the presence of various doses of Eleutherococcus preparation in RPMI-1640 medium with 10% fetal serum then used as effectors in the membranotoxic text and the level of proliferation was determined.
- Research Organization:
- Scientific Research Institute for the Biological Testing of Chemical Compounds, Kupavna (USSR)
- OSTI ID:
- 6270777
- Journal Information:
- Pharmaceut. Chem. J.; (United States), Vol. 20:8; Other Information: Translated from Khimiko-Farmatsevticheski Zhurnal; 20: No. 8, 914-917(Aug 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLYCOSIDES
BIOLOGICAL EFFECTS
TRACER TECHNIQUES
INTERFERON
BIOSYNTHESIS
LYMPHOKINES
SPLEEN CELLS
CELL PROLIFERATION
ANTI-INFECTIVE AGENTS
ANTINEOPLASTIC DRUGS
BACTERIA
BIOCHEMICAL REACTION KINETICS
CELL KILLING
CONCANAVALIN
CYTOCHEMISTRY
METABOLISM
MICE
PHARMACOLOGY
STIMULATION
THYMIDINE
TOXICITY
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AZINES
BIOCHEMISTRY
CARBOHYDRATES
CHEMISTRY
DRUGS
GROWTH FACTORS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LECTINS
MAMMALS
MICROORGANISMS
MITOGENS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550501* - Metabolism- Tracer Techniques
550301 - Cytology- Tracer Techniques
560300 - Chemicals Metabolism & Toxicology