The effects of different schedules of total-body irradiation in heterotopic vascularized bone transplantation. An experimental study in the Lewis rat

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Transplantation; (USA)

DOI:https://doi.org/10.1097/00007890-199012000-00003· OSTI ID:6270328

Gonzalez del Pino, J; Benito, M; Randolph, M A; Weiland, A J ^[1]

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1. Johns Hopkins Hospital, Baltimore, MD (USA)

To evaluate the effects of irradiation on heterotopically placed vascularized knee isografts, a single dose of 10 Gy of total-body irradiation was given to Lewis donor rats. Irradiation was delivered either 2 or 6 days prior to harvesting or subsequent transplantation, and evaluated at 1, 2, and 4 weeks after grafting. Irradiation caused endothelial depopulation of the graft artery, although vascular pedicle patency was maintained throughout the study. Bone graft viability and mineralization were normal. Dramatic changes in the bone marrow were seen that included an increase of its fat content (P less than 0.001), and a concomitant decrease in bone marrow-derived immunocompetent cells. These changes were more prominent in recipients of grafts from day -6 irradiated donor rats. Total-body irradiation did not prejudice the use of vascularized bone grafts, and exhibited an associated immunosuppresant effect over the vascular endothelium and bone marrow. This may be a further rational conditioning procedure to avoid recipient manipulation in vascularized bone allotransplantation.

• OSTI ID: 6270328
• Journal Information: Transplantation; (USA), Vol. 50:6; ISSN 0041-1337
• Country of Publication: United States
• Language: English

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