Characterization of the cellulolytic activity of a bacillus isolate
A group I Bacillus strain, DLG, was isolated and characterized as being most closely related to Bacillus subtilis. When grown on any of a variety of sugars, the culture supernatant of this isolate was found to process cellulolytic activity, as demonstrated by degradation of trinitrophenyl-carboxymethyl cellulose. Growth in medium containing cellobiose or glucose resulted in the greatest production of cellulolytic activity. The cellulolytic activity was not produced until the stationary phase of growth, and the addition of glucose or cellobiose to a culture in this phase had no apparent effect on enzyme production. Fractionation of the culture supernatant showed that the molecular weight of the enzymatic activity was less than 100,000. Maximum cellulolytic activity in assays was observed at pH 4.8 and at 58 degrees C, although maximum thermal stability of the activity was observed only up to 45 to 50 degrees C. Neither glucose nor cellobiose inhibited enzymatic activity. Kinetic experiments suggested that more than one enzyme was acting upon trinitrophenyl-carboxymethyl cellulose. Exocellular protein produced by this Bacillus isolate showed roughly one-fifth the cellulolytic activity displayed by Trichoderma reesei C30 on noncrystalline cellulosic substrates. In contrast to T. reesei cellulase, the Bacillus enzymatic activity showed no ability to degrade crystalline forms of cellulose, nor was cellobiase activity detectable. 32 references.
- Research Organization:
- Univ. of Wisconsin, Madison, WI
- OSTI ID:
- 6269368
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 47:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
BACILLUS
CELLULOLYTIC ACTIVITY
BIOCONVERSION
CELLOBIOSE
CELLULASE
COMPARATIVE EVALUATIONS
MOLECULAR WEIGHT
REACTION KINETICS
SPECIFICATIONS
SUBSTRATES
TRICHODERMA VIRIDE
BACTERIA
CARBOHYDRATES
DISACCHARIDES
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
KINETICS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
TRICHODERMA
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