Acrolein stimulates eicosanoid release from bovine airway epithelial cells
- Univ. of Cincinnati College of Medicine, OH (USA)
Injury to the airway mucosa after exposure to environmental irritants is associated with pulmonary inflammation and bronchial hyperresponsiveness. To better understand the relationships between mediator release and airway epithelial cell injury during irritant exposures, we studied the effects of acrolein, a low-molecular-weight aldehyde found in cigarette smoke, on arachidonic acid metabolism in cultured bovine tracheal epithelial cells. Confluent airway epithelial cell monolayers, prelabeled with (3H)arachidonic acid, released significant levels of 3H activity when exposed (20 min) to 100 microM acrolein. (3H)arachidonic acid products were resolved using reverse-phase high-performance liquid chromatography. Under control conditions the released 3H activity coeluted predominantly with the cyclooxygenase product, prostaglandin (PG) E2. After exposure to acrolein, significant peaks in 3H activity coeluted with the lipoxygenase products 12-hydroxyeicosatetraenoic acid (HETE) and 15-HETE, as well as with PGE2, PGF2 alpha, and 6-keto-PGF1 alpha. Dose-response relationships for acrolein-induced release of immunoreactive PGF2 alpha and PGE2 from unlabeled epithelial monolayers demonstrated 30 microM acrolein as the threshold dose, with 100 microM acrolein inducing nearly a fivefold increase in both PGF2 alpha and PGE2. Cellular viability after exposure to 100 microM acrolein, determined by released lactate dehydrogenase activity, was not affected until exposure periods were greater than or equal to 2 h. These results implicate the airway epithelial cell as a possible source of eicosanoids after exposure to acrolein.
- OSTI ID:
- 6267622
- Journal Information:
- American Journal of Physiology; (USA), Vol. 259:4 Pt 1; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACROLEIN
BIOLOGICAL EFFECTS
ARACHIDONIC ACID
METABOLISM
EICOSANOIC ACID
BIOSYNTHESIS
RESPIRATORY SYSTEM DISEASES
PATHOGENESIS
CATTLE
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
EPITHELIUM
LACTATE DEHYDROGENASE
LIQUID COLUMN CHROMATOGRAPHY
PROSTAGLANDINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DISEASES
DOMESTIC ANIMALS
ENZYMES
HEMIACETAL DEHYDROGENASES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RUMINANTS
SEPARATION PROCESSES
SYNTHESIS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques