Effect of supra-ambient oxygen on nitrogenase activity (C/sub 2/H/sub 2/) and root respiration of soybeans and isolated soybean bacteroids

Journal Article · Sat Jan 01 00:00:00 EST 1983 · Plant Physiol.; (United States)

OSTI ID:6263942

Patterson, T G; Peterson, J B; LaRue, T A

Isolated soybean (Glycine max (L.) Merr. cv Wilkin) bacteroids have O/sub 2/-dependent nitrogenase activity which is strongly inhibited by supraoptimal O/sub 2/ concentrations. Oxygen-inhibited nitrogenase activity is recovered by addition of 10 millimolar sodium succinate or by lowering the O/sub 2/ concentration. Brief treatment of roots of intact soybean plants with 1.0 atmosphere O/sub 2/ reduces nitrogenase activity (C/sub 2/H/sub 2/). There is a rapid partial recovery of activity within 2 to 3 hours, and a slower return to near normal levels by 36 hours. The drop and recovery of nitrogenase activity is accompanied by a parallel drop and increase in root respiration. There is a direct relationship between the change in respiration and the change in acetylene reduction following O/sub 2/ treatment. The O/sub 2/-mediated changes in nitrogenase activity and root respiration are not affected by the planting medium. The ratio of the change in respiration to the change in nitrogenase activity was the same in 13 soybean cultivars.

• Research Organization: Boyce Thompson Inst., Ithaca, NY
• DOE Contract Number: AC02-82ER12066
• OSTI ID: 6263942
• Journal Information: Plant Physiol.; (United States), Vol. 70
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLYCINE HISPIDA
RESPIRATION
NITROGENASE
INACTIVATION
OXYGEN
BIOLOGICAL EFFECTS
ROOTS
ENZYME ACTIVITY
BIOLOGICAL RECOVERY
ENZYME INHIBITORS
ELEMENTS
ENZYMES
LEGUMINOSAE
NITRO-GROUP DEHYDROGENASES
NONMETALS
OXIDOREDUCTASES
PLANTS
RECOVERY
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)