Fatty acid-binding protein activities in bovine muscle, liver and adipose tissue

Journal Article · Fri Nov 01 00:00:00 EST 1985 · J. Nutr.; (United States)

OSTI ID:6259447

Smith, S B; Ekeren, P A; Sanders, J O

Subcutaneous adipose tissue, sternomandibularis muscle and liver were obtained from steers immediately postmortem. Muscle strips and adipose tissue snips were incubated with 0.75 mM (1- UC)palmitate and 5 mM glucose. Muscle strips esterified palmitate at the rate of 2.5 nmol/min per gram tissue, which was 30% of the rate observed for adipose tissue. Fatty acid-binding protein activity was measured in 104,000 x g supernatant fractions of liver, muscle and adipose tissue homogenates. Muscle and adipose tissue fractions bound 840 and 140 pmol (1- UC)palmitoyl-CoA per gram tissue, respectively. Fatty acid-binding protein activity was greater in adipose tissue than in muscle when data were expressed per milligram protein. Fatty acid binding-protein activity was correlated with the rate of palmitate esterification within each tissue. Liver contained the highest fatty acid-binding protein activity.

• Research Organization: Texas A and M Univ., College Station
• OSTI ID: 6259447
• Journal Information: J. Nutr.; (United States), Vol. 11
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ESTERIFICATION
HEXADECANOIC ACID
ADIPOSE TISSUE
CATTLE
COENZYMES
DIET
GLUCOSE
LIVER
MUSCLES
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
GLANDS
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RUMINANTS
SACCHARIDES
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques