Nitrogen-15 NMR studies of solid dipeptides

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6257306

Hartzell, C J

The first part of this study deals with the systematic determination of the /sup 15/N chemical shift tensor for the series of dipeptides N-acetyl(1-/sup 13/C)-glycyl-(/sup 15/N)-X-amide (GlyX: X = alanine, glycine, tyrosine), (1-/sup 13/C)-glycyl(/sup 15/N)-glycine/center dot/HCL and (1-/sup 13/C)-ananyl-(/sup 15/N)-alanine (AlaAla). The principal values and polar angles relating the chemical shift tensor to the peptide C-N bond are given for all dipeptides studied. The isotropic values for /sup 15/N varied from 105 ppm up to 119.7ppm for all the dipeptides studied and the angle ..beta../sub CN/ relating sigma/sub 33/to the C-N bond varied from 98/degree/ to 106/degree/. GlyAla and AlaAla showed striking differences in asymmetry parameter, .37 vs. .13, and anisotropy, 165 vs. 144, yet the isotropic values for these two are identical. The /sup 13/C chemical shift principal values and polar angles are determined for AlaAla. The second part of the study deals with an experiment to determine unambiguously the orientation of the chemical shift tensor in the molecular frame.

• Research Organization: Washington Univ., Seattle (USA)
• OSTI ID: 6257306
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
CHEMICAL SHIFT
CARBON 13
MOLECULAR STRUCTURE
NITROGEN 15
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
TRACER TECHNIQUES
CARBON ISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RESONANCE
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques