Plasmodium falciparum: assessment of in vitro growth by (/sup 3/H)hypoxanthine incorporation
To evaluate rapidly Plasmodium falciparum growth in Vitro, (/sup 3/H)hypoxanthine was added to parasite microcultures and radioisotope incorporation was measured. When culture parameters were carefully controlled, (/sup 3/H)hypoxanthine incorporation was proportional to the number of parasitized erythrocytes present. Factors affecting (/sup 3/H)hypoxanthine incorporation included initial parasitemia, duration of culture, duration of radioisotope pulse, parasite stage, concentration of uninfected erythrocytes, the use of serum or plasma to supplement growth, and the concentration of a variety of purines in the culture medium. The method described can be used to measure inhibition of P. falciparum growth by immune serum and has previously been used to study antimalarial drug activity in vitro.
- Research Organization:
- Department of Immunology, Walter Reed Army Institute of Research, Washington, DC
- OSTI ID:
- 6255349
- Journal Information:
- Exp. Parasitol.; (United States), Vol. 55:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HYPOXANTHINE
METABOLISM
PLASMODIUM
GROWTH
CELL CULTURES
COMPARATIVE EVALUATIONS
ERYTHROCYTES
IMMUNOLOGY
MEASURING METHODS
QUANTITY RATIO
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PROTOZOA
PURINES
SPOROZOA
550701* - Microbiology- Tracer Techniques